AB108391

Anti-Cripto1/CRIPTO antibody [EPNCIR106A]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal Cripto1/CRIPTO antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF

5 Images

Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Intracellular Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling Cripto1/CRIPTO with purified ab108391at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1/CRIPTO with Purified ab108391 at 1 : 500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Lab

Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

ab108391 (purified) at 1 : 30 dilution (2ug) immunoprecipitating Cripto1/CRIPTO in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
Lane 1 (input) : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.

All lanes:

Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)

Predicted band size: 21 kDa

false

Lab

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/2000 dilution

All lanes:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 21 kDa

false

Unknown

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

All lanes:

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/1000 dilution

All lanes:

NCCIT cell lysate at 10 µg

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

578 PE

PE Anti-Cripto1/CRIPTO antibody [EPNCIR106A]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR106A

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "For unpurified use at 1/100 -1/250.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Allow to warm to room temp and agitate gently before aliquoting

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed : 27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed : 11909953).

See full target information CRIPTO

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:819682 PubMed35222539

2022

Defining the Pluripotent Marker Genes for Identification of Teleost Fish Cell Pluripotency During Reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Huajin Li,Wenting Xu,Sijia Xiang,Leiting Tao,Wen Fu,Jinhui Liu,Wenbin Liu,Yamei Xiao,Liangyue Peng

Frontiers in oncology 11:730615 PubMed34434900

2021

Exosomal Cripto-1 Serves as a Potential Biomarker for Perihilar Cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chunxiao Hu,Yanli Zhang,Mengjiao Zhang,Tingting Li,Xin Zheng,Qining Guo,Xin Zhang

Annals of translational medicine 9:485 PubMed33850882

2021

MiR-138-1-3p alters the stemness and radiosensitivity of tumor cells by targeting CRIPTO and the JAK2/STAT3 pathway in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Du,Jiahui Jiang,Yiting Chen,Nengwei Zhang,Guanyang Chen,Xingwei Wang,Xueying Long,Xueping Feng

Journal of cellular biochemistry 121:545-556 PubMed31310365

2019

An evaluation of different Cripto-1 antibodies and their variable results.

Applications

Unspecified application

Species

Unspecified reactive species

Johann Mar Gudbergsson,Meg Duroux

Cell death and differentiation 25:1426-1441 PubMed29445127

2018

Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Regina Cheuk-Lam Lo,Carmen Oi-Ning Leung,Kristy Kwan-Shuen Chan,Daniel Wai-Hung Ho,Chun-Ming Wong,Terence Kin-Wah Lee,Irene Oi-Lin Ng

The Journal of biological chemistry 292:4138-4151 PubMed28126904

2017

Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic.

Applications

Unspecified application

Species

Unspecified reactive species

Senem Aykul,Anthony Parenti,Kit Yee Chu,Jake Reske,Monique Floer,Amy Ralston,Erik Martinez-Hackert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cripto1/CRIPTO antibody [EPNCIR106A]

Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise