Rabbit Recombinant Monoclonal Cripto1/CRIPTO antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.
Images
![Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--flow-cytometry-intracellular-img25610.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)")
![Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--immunoprecipitation-img123.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)")
![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--western-blot-img145099.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--immunocytochemistry-immunofluorescence-img18691.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)")
![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--western-blot-img87989.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (AB108391)")
Publications
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- Frontiers in genetics 13:8196822022Defining the Pluripotent Marker Genes for Identification of Teleost Fish Cell Pluripotency During Reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuajin Li,Wenting Xu,Sijia Xiang,Leiting Tao,Wen Fu,Jinhui Liu,Wenbin Liu,Yamei Xiao,Liangyue PengPubMed 35222539
- Frontiers in oncology 11:7306152021Exosomal Cripto-1 Serves as a Potential Biomarker for Perihilar Cholangiocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunxiao Hu,Yanli Zhang,Mengjiao Zhang,Tingting Li,Xin Zheng,Qining Guo,Xin ZhangPubMed 34434900
- Annals of translational medicine 9:4852021MiR-138-1-3p alters the stemness and radiosensitivity of tumor cells by targeting CRIPTO and the JAK2/STAT3 pathway in nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Du,Jiahui Jiang,Yiting Chen,Nengwei Zhang,Guanyang Chen,Xingwei Wang,Xueying Long,Xueping FengPubMed 33850882
- Journal of cellular biochemistry 121:545-5562019An evaluation of different Cripto-1 antibodies and their variable results.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohann Mar Gudbergsson et. al.PubMed 31310365
- Cell death and differentiation 25:1426-14412018Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRegina Cheuk-Lam Lo et. al.PubMed 29445127
- The Journal of biological chemistry 292:4138-41512017Biochemical and Cellular Analysis Reveals Ligand Binding Specificities, a Molecular Basis for Ligand Recognition, and Membrane Association-dependent Activities of Cripto-1 and Cryptic.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSenem Aykul et. al.PubMed 28126904
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes For unpurified use at 1/100 -1/250. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated CRIPTO acts as a Nodal coreceptor in cis. Shedding of CRIPTO by TMEM8A modulates Nodal signaling by allowing soluble CRIPTO to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953).
Alternative names
CRIPTO-1, TDGF1, CRIPTO, Protein Cripto, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1, CRGF
Recommended products
Rabbit Recombinant Monoclonal Cripto1/CRIPTO antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPNCIR106A
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Allow to warm to room temp and agitate gently before aliquoting
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--flow-cytometry-intracellular-img25610.jpg "Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)")
Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)
Intracellular Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling Cripto1/CRIPTO with purified ab108391at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
![Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--immunoprecipitation-img123.jpg "Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)")
Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)
ab108391 (purified) at 1:30 dilution (2ug) immunoprecipitating Cripto1/CRIPTO in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.All lanes: Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)
Predicted band size: 21 kDa
![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--western-blot-img145099.jpg "Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)")
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/2000 dilution
All lanes: NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 21 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--immunocytochemistry-immunofluorescence-img18691.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)")
Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)
Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1/CRIPTO with Purified ab108391 at 1:500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391), expandable thumbnail](https://content.abcam.com/products/images/cripto1-cripto-antibody-epncir106a-ab108391--western-blot-img87989.jpg "Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)")
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)
All lanes: Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/1000 dilution
All lanes: NCCIT cell lysate at 10 µg
Predicted band size: 21 kDa
Observed band size: 21 kDa
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