Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (AB247653)

This data was developed using ab108391, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling Cripto1/CRIPTO with purified ab108391 at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (AB247653)

This data was developed using ab108391, the same antibody clone in a different buffer formulation.

Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1/CRIPTO with Purified ab108391 at 1 : 500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IP

Lab

Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (AB247653)

This data was developed using ab108391, the same antibody clone in a different buffer formulation.

ab108391 (purified) at 1 : 30 dilution (2ug) immunoprecipitating Cripto1/CRIPTO in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
Lane 1 (input) : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.

All lanes:

Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (<a href='/en-us/products/primary-antibodies/cripto1-cripto-antibody-epncir106a-ab108391'>ab108391</a>)

Predicted band size: 21 kDa

false

• WB

Lab

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (AB247653)

This data was developed using ab108391, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (<a href='/en-us/products/primary-antibodies/cripto1-cripto-antibody-epncir106a-ab108391'>ab108391</a>) at 1/2000 dilution

All lanes:

NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 21 kDa

false

• WB

Unknown

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (AB247653)

This data was developed using ab108391, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (<a href='/en-us/products/primary-antibodies/cripto1-cripto-antibody-epncir106a-ab108391'>ab108391</a>) at 1/1000 dilution

All lanes:

NCCIT cell lysate at 10 µg

Predicted band size: 21 kDa

Observed band size: 21 kDa

false