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Mouse Recombinant Monoclonal CRISPR-associated endonuclease Cas12a antibody. Suitable for WB and reacts with Transfected cell lysate - Acidaminococcus sp. samples.

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Images

Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (AB273438), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Acidaminococcus sp.
Predicted
Transfected cell lysate - Acidaminococcus sp.
Tested

Tested
Tested

Species
Transfected cell lysate - Acidaminococcus sp.
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Acidaminococcus sp.
Dilution info
-
Notes

-

Target data

Function

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed:26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed:26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed:27114038). Protects E.coli against plasmids and bacteriophage M13mp18, phage T4 with hydroxymethyl or unmodified (but not glycosylated) cytosines and to a lesser extent against lambda and VpaE1 phage (PubMed:38261981). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity).

Alternative names

cpf1, HMPREF1246_0236, cas12a, CRISPR-associated endonuclease Cas12a, AsCpf1, CRISPR-associated endonuclease Cpf1

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Mouse Recombinant Monoclonal CRISPR-associated endonuclease Cas12a antibody. Suitable for WB and reacts with Transfected cell lysate - Acidaminococcus sp. samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
3D3-F7
Purification technique
Affinity purification Protein A
Specificity

Reacts with Acidaminococcus sp.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CRISPR-associated endonuclease Cas12a also known as Cpf1 is a single-effector protein used in genome editing. This protein has a molecular weight around 150 kDa and is found in various bacterial species as a defense mechanism against viral infections. Cas12a performs its function by creating double-strand breaks in DNA through its endonuclease activity. It recognizes specific DNA sequences via a guide RNA and cleaves the DNA allowing for precise genetic modifications.

Biological function summary

Cas12a serves an important role in the CRISPR-Cas adaptive immune system in bacteria. It is part of the Class 2 CRISPR systems and functions in a complex with a single guide RNA (sgRNA). In this complex Cas12a distinguishes itself from other CRISPR-associated proteins by requiring a simpler PAM sequence which offers greater flexibility in target DNA recognition. Cas12a's ability to cleave both strands of DNA with precision makes it a valuable tool for genetic engineering and functional genomics studies.

Pathways

Cas12a enables targeted edits in genetic pathways contributing to advancements in synthetic biology and gene therapy applications. Cas12a interacts with the DNA repair machinery influencing pathways like non-homologous end joining (NHEJ) and homology-directed repair (HDR). These pathways are also influenced by proteins such as DNA ligase IV in NHEJ and RAD51 in HDR showcasing how Cas12a-mediated DNA modifications are repaired and integrated.

Associated diseases and disorders

Cas12a has potential applications in treating genetic disorders through targeted gene correction or disruption. Cas12a can contribute to research and therapeutic strategies for diseases like sickle cell anemia and muscular dystrophy where precise genome editing can address genetic defects. This system related to human proteins involved in DNA repair pathways may offer innovative solutions in correcting disease-causing mutations and restoring normal cellular functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (ab273438), expandable thumbnail

    Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (ab273438)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (ab273438) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: HEK-293T transfected with AsCpf1 Cas12a expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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