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AB273478

Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free

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Mouse Recombinant Monoclonal CRISPR-associated endonuclease Cas12a antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Acidaminococcus sp. samples.

View Alternative Names

cpf1, HMPREF1246_0236, cas12a, CRISPR-associated endonuclease Cas12a, AsCpf1, CRISPR-associated endonuclease Cpf1

1 Images
Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (AB273478)
  • WB

Supplier Data

Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] - BSA and Azide free (AB273478)

This image was produced using ab273438, the same clone but in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7] (<a href='/en-us/products/primary-antibodies/crispr-associated-endonuclease-cas12a-antibody-3d3-f7-ab273438'>ab273438</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with AsCpf1 Cas12a expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 3s

  • Unconjugated

    Anti-CRISPR-associated endonuclease Cas12a antibody [3D3-F7]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3D3-F7

Isotype

IgG2a

Carrier free

Yes

Reacts with

Acidaminococcus sp.

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with Acidaminococcus sp.

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Complementary Products

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Sample Prep & Detection Kits

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Acidaminococcus sp.": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Acidaminococcus sp.": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab273478 is the carrier-free version of ab273438.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRISPR-associated endonuclease Cas12a also known as Cpf1 is a single-effector protein used in genome editing. This protein has a molecular weight around 150 kDa and is found in various bacterial species as a defense mechanism against viral infections. Cas12a performs its function by creating double-strand breaks in DNA through its endonuclease activity. It recognizes specific DNA sequences via a guide RNA and cleaves the DNA allowing for precise genetic modifications.
Biological function summary

Cas12a serves an important role in the CRISPR-Cas adaptive immune system in bacteria. It is part of the Class 2 CRISPR systems and functions in a complex with a single guide RNA (sgRNA). In this complex Cas12a distinguishes itself from other CRISPR-associated proteins by requiring a simpler PAM sequence which offers greater flexibility in target DNA recognition. Cas12a's ability to cleave both strands of DNA with precision makes it a valuable tool for genetic engineering and functional genomics studies.

Pathways

Cas12a enables targeted edits in genetic pathways contributing to advancements in synthetic biology and gene therapy applications. Cas12a interacts with the DNA repair machinery influencing pathways like non-homologous end joining (NHEJ) and homology-directed repair (HDR). These pathways are also influenced by proteins such as DNA ligase IV in NHEJ and RAD51 in HDR showcasing how Cas12a-mediated DNA modifications are repaired and integrated.

Cas12a has potential applications in treating genetic disorders through targeted gene correction or disruption. Cas12a can contribute to research and therapeutic strategies for diseases like sickle cell anemia and muscular dystrophy where precise genome editing can address genetic defects. This system related to human proteins involved in DNA repair pathways may offer innovative solutions in correcting disease-causing mutations and restoring normal cellular functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Recognizes a short motif in the CRISPR repeat sequences (the 5' PAM or protospacer adjacent motif, TTTN in this organism) to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs (PubMed : 26422227). Has dsDNA endonuclease activity, results in staggered 4-base 5' overhangs 19 and 22 bases downstream of the PAM on the non-targeted and targeted strand respectively (PubMed : 26422227). Non-target strand cleavage by the RuvC domain is probably a prerequisite of target strand cleavage by the Nuc domain (PubMed : 27114038). Protects E.coli against plasmids and bacteriophage M13mp18, phage T4 with hydroxymethyl or unmodified (but not glycosylated) cytosines and to a lesser extent against lambda and VpaE1 phage (PubMed : 38261981). In this CRISPR system correct processing of pre-crRNA requires only this protein and the CRISPR locus (By similarity).
See full target information cas12a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com