AB315192

Anti-CRISPR-Cas9 antibody [6F7]

Name: Anti-CRISPR-Cas9 antibody [6F7] (ab315192) | Abcam
Brand: Abcam Limited
SKU: AB315192
Price: 580 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CRISPR-Cas9 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Staphylococcus aureus, Transfected cell line - Staphylococcus aureus samples. Cited in 1 publication. Immunogen corresponding to Native Fragment Protein within Staphylococcus aureus CRISPR-associated endonuclease Cas9.

View Alternative Names

CRISPR-associated endonuclease Cas9, SaCas9, cas9

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [6F7] (AB315192)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [6F7] (AB315192)

HEK293 cells were transfected with a fusion construct of green fluorescent protein (GFP) fused with the C-terminal sequence 803-1053 of S. aureus Cas9. Cells were stained with ab315192, in red and the expressed GFP gives a green signal. The two signals overlap as expected. The nuclei of both transfected and untransfected cells are revealed by the DAPI DNA stain.

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody [6F7] (AB315192)

Western blot analysis of HEK293 cell lysates using ab315192 : [1] protein standard (red), [2] non-transfected cells, and [3] transfected cells with GFP-Cas9 (C-terminal 803-1053 amino acids of S. aureus CAS9) fusion conctruct. Strong band at about 53kDa corresponds to the GFP-CAS9 fusion protein.

All lanes:

Western blot - Anti-CRISPR-Cas9 antibody [6F7] (ab315192)

Lane 2:

non-transfected HEK293 cell lysate

Lane 3:

transfected HEK293 cell lysate with GFP-Cas9 (C-terminal 803-1053 amino acids of S. aureus CAS9) fusion conctruct

Observed band size: 53 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6F7

Isotype

IgG1

Carrier free

Reacts with

Staphylococcus aureus

Applications

ICC/IF, WB

applications

Immunogen

Native Fragment Protein within Staphylococcus aureus CRISPR-associated endonuclease Cas9.

J7RUA5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Staphylococcus aureus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Staphylococcus aureus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.

Biological function summary

The Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.

Pathways

CRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.

CRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.

See full target information CRISPR-associated endonuclease Cas9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102046 PubMed40239628

2025

Specific macrophage RhoA targeting CRISPR-Cas9 for mitigating osteoclastogenesis-induced joint damage in inflammatory arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhai Chen,Jianwei Tan,Nannan Wang,Hui Li,Wenxiang Cheng,Jian Li,Benguo Wang,Adam C Sedgwick,Zhitong Chen,Guojun Chen,Peng Zhang,Wei Zheng,Chengbo Liu,Jingqin Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CRISPR-Cas9 antibody [6F7]

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [6F7] (AB315192)

Western blot - Anti-CRISPR-Cas9 antibody [6F7] (AB315192)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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