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Rabbit Monoclonal CRISPR-Cas9 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Streptococcus pyogenes, Transfected cell lysate - Streptococcus thermophilus, Transfected cell lysate - Neisseria meningitidis samples. Cited in 2 publications.

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Images

Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (AB210752), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (AB210752), expandable thumbnail
  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (AB210752), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (AB210752), expandable thumbnail
  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (AB210752), expandable thumbnail

Publications

  • Molecular cancer research : MCR 18:891-9022020
    Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Kelly Banas et. al.
    PubMed 32184217
  • Parasites & vectors 10:2812017
    Identification and validation of a Schistosoma japonicum U6 promoter.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Qing Li et. al.
    PubMed 28583151

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Neisseria meningitidis
Predicted
Predicted
Streptococcus pyogenes
Predicted
Predicted
Streptococcus thermophilus
Predicted
Predicted
Transfected cell lysate - Neisseria meningitidis
Tested
Not recommended
Transfected cell lysate - Streptococcus pyogenes
Tested
Not recommended
Transfected cell lysate - Streptococcus thermophilus
Tested
Tested

Tested
Tested

Species

Transfected cell lysate - Streptococcus pyogenes

Dilution info

1/5000

Notes

-

Species

Transfected cell lysate - Streptococcus thermophilus

Dilution info

1/5000

Notes

-

Species

Transfected cell lysate - Neisseria meningitidis

Dilution info

1/5000

Notes

-

Predicted
Predicted

Species

Neisseria meningitidis, Streptococcus pyogenes, Streptococcus thermophilus

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell lysate - Streptococcus thermophilus

Dilution info

1/50

Notes

-

Predicted
Predicted

Species

Neisseria meningitidis, Streptococcus pyogenes, Streptococcus thermophilus

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Streptococcus pyogenes, Transfected cell lysate - Neisseria meningitidis

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (Probable). Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer yielding blunt ends; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes a 3'-G-rich protospacer adjacent motif (PAM, TGGTG in this organism) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming.

      Alternative names

      CRISPR-associated endonuclease Cas9, St-Cas9, csn1, cas9

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      Rabbit Monoclonal CRISPR-Cas9 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Streptococcus pyogenes, Transfected cell lysate - Streptococcus thermophilus, Transfected cell lysate - Neisseria meningitidis samples. Cited in 2 publications.

      Alternative names

      CRISPR-associated endonuclease Cas9, St-Cas9, csn1, cas9

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR19619-92

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      CRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.

      Biological function summary

      The Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.

      Pathways

      CRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.

      Associated diseases and disorders

      CRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      5 product images

      • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752), expandable thumbnail

        Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752) at 1/5000 dilution

        Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal) with GFP-tag at 20 µg

        Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

        Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

        Lane 4: Rat embryo lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 164 kDa

        Exposure time: 3s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected with Streptococcus Thermophilus CRISPR-Cas9 (pcDNA3.1(+)-Myc-His) with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.

        Positive staining on the 293T cells transfected with Streptococcus Thermophilus Cas9.

      • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752), expandable thumbnail

        Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        The immunogen is located at the N-terminus.

        All lanes: Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752) at 1/5000 dilution

        Lane 1: Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control) at 20 µg

        Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-tag at 20 µg

        Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-tag at 20 µg

        Lane 4: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-tag at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 164 kDa

        Exposure time: 5s

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected blank pcDNA3.1(+)-Myc-His vector with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.

        Negative on the 293T cells transfected blank pcDNA3.1(+)-Myc-His vector.

      • Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752), expandable thumbnail

        Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        Exposure time: Lane 1-2: 5 seconds; Lane 3: 3 minutes.

        All lanes: Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] (ab210752) at 1/5000 dilution

        Lane 1: Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control) at 20 µg

        Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491/LMD-9)) with His-tag at 20 µg

        Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A) with His-tag at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 164 kDa

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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