Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (AB223548)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected with Streptococcus Thermophilus CRISPR-Cas9 (pcDNA3.1(+)-Myc-His) with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.

Positive staining on the 293T cells transfected with Streptococcus Thermophilus Cas9.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210752).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (AB223548)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected blank pcDNA3.1(+)-Myc-His vector with ab210752 at 1/50 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.

Negative on the 293T cells transfected blank pcDNA3.1(+)-Myc-His vector.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210752).

• WB

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (AB223548)

Blocking/Dilution buffer : 5% NFDM/TBST.

The immunogen is located at the N-terminus.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210752).

All lanes:

Western blot - Anti-CRISPR-Cas9 antibody [EPR19619-92] - BSA and Azide free (ab223548) at 1/5000 dilution

Lane 1:

Untransfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate (control) at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-tag at 20 µg

Lane 3:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-tag at 20 µg

Lane 4:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-tag at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 164 kDa

false