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Rabbit Recombinant Monoclonal CRISPR-Cas9 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Streptococcus thermophilus samples. Cited in 1 publication.

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Images

Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (AB202657), expandable thumbnail
  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (AB202657), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19633] (AB202657), expandable thumbnail
  • Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] (AB202657), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19633] (AB202657), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Streptococcus thermophilus
Predicted
Predicted
Predicted
Predicted
Transfected cell lysate - Streptococcus thermophilus
Tested
Tested
Tested
Tested

Tested
Tested

Species
Transfected cell lysate - Streptococcus thermophilus
Dilution info
1/30
Notes

-

Predicted
Predicted

Species
Streptococcus thermophilus
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Streptococcus thermophilus
Dilution info
1/20000
Notes

-

Predicted
Predicted

Species
Streptococcus thermophilus
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Streptococcus thermophilus
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Streptococcus thermophilus
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Streptococcus thermophilus
Dilution info
1/60
Notes

-

Predicted
Predicted

Species
Streptococcus thermophilus
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (Probable). Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer yielding blunt ends; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes a 3'-G-rich protospacer adjacent motif (PAM, TGGTG in this organism) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity. When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming.

Additional Targets

CRISPR-Cas9

Alternative names

csn1, cas9, CRISPR-associated endonuclease Cas9, St-Cas9

Recommended products

Rabbit Recombinant Monoclonal CRISPR-Cas9 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Streptococcus thermophilus samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19633
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.

Biological function summary

The Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.

Pathways

CRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.

Associated diseases and disorders

CRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657), expandable thumbnail

    Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657) at 1/20000 dilution

    Lane 1: Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag at 20 µg

    Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, N–terminal aa1-800) with GFP-Myc tag at 20 µg

    Lane 4: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag at 20 µg

    Lane 5: Empty vector with Myc-His tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 6: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) with Myc-His tag at 20 µg

    Lane 7: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag at 20 µg

    Lane 8: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)) with Myc-His tag at 20 µg

    Lane 9: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 100000 Cells

    Predicted band size: 164 kDa

    Exposure time: 5s

  • Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657), expandable thumbnail

    Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657) at 1/20000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, C–terminal aa801-1409) with GFP-Myc tag at 20 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Lane 4: Rat embryo lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 164 kDa

    Observed band size: 86 kDa

    Exposure time: 5s

  • Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag or Empty vector, labeling CRISPR-Cas9 with ab202657 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) secondary antibody at 1/1000 dilution.

    Confocal image showing Positive staining on HeLa cells transfected with CRISPR-Cas9(G3ECR1, Streptococcus thermophilus) with Myc-His tag.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab202657 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657), expandable thumbnail

    Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657)

    CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag with ab202657 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab202657 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag 10μg (Input).

    Lane 2: ab202657 IP in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab202657 in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657)

  • Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19633] (ab202657)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag labeling CRISPR-Cas9 with ab202657 at 1/60 dilution (red) compared with a RabbitIgG,monoclonal [EPR25A]-Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Goat anti Rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

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