Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (AB251383)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag or Empty vector, labeling CRISPR-Cas9 with ab202657 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150079) secondary antibody at 1/1000 dilution.

Confocal image showing Positive staining on HeLa cells transfected with CRISPR-Cas9(G3ECR1, Streptococcus thermophilus) with Myc-His tag.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab202657 at 1/100 dilution followed by ab150120 at 1/1000 dilution.

-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202657).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (AB251383)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag labeling CRISPR-Cas9 with ab202657 at 1/60 dilution (red) compared with a RabbitIgG,monoclonal [EPR25A]-Isotype control (ab172730) (black). Goat anti Rabbit IgG (Alexa Fluor^®488) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202657).

• IP

Supplier Data

Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] - BSA and Azide free (AB251383)

CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag with ab202657 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab202657 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag 10μg (Input).

Lane 2 : ab202657 IP in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag.

Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab202657 in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202657).

All lanes:

Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19633] (<a href='/en-us/products/primary-antibodies/crispr-cas9-antibody-epr19633-ab202657'>ab202657</a>)

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