AB203943

Anti-CRISPR-Cas9 antibody [EPR19795]

20ul selling size
RabMAb
Recombinant
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(5 Publications)

Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) is a rabbit monoclonal antibody detecting CRISPR-Cas9 in Western Blot, Flow Cytometry (Intra), IP, ICC/IF. Suitable for Staphylococcus aureus.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CRISPR-associated endonuclease Cas9, SaCas9, cas9, CRISPR-Cas9/Csn1, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9

6 Images

Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector,labeling CRISPR-Cas9 with ab203943 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black). Goat anti rabbit IgG (Alexa Fluorr^®647) at 1/2000 dilution was used as the secondary antibody.

Note : The image is obtained by gating the GFP-tag positive population.

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CRISPR-Cas9 with ab203943 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing positive staining on HEK-293T cells transfected with a GFP-tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) expression construct.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector, with ab203943 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203943 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector, 10 μg (Input).

Lane 2 : ab203943 IP in HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203943 in HEK-293T whole cell lysate transfected with a GFP tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943)

Observed band size: 150 kDa

false

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) at 1/5000 dilution

Lane 1:

HEK-293T whole cell lysate (Human epithelial cell line from embryonic kidney) transfected with an empty vector containing Myc-His tag (vector control) at 20 µg

Lane 2:

HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (A1IQ68, Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)) vector at 20 µg

Lane 3:

HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) vector at 20 µg

Lane 4:

HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (Q03JI6, Streptococcus thermophiles (strain ATCC BAA-491/ LMD-9)) vector at 20 µg

Lane 5:

HEK-293T whole cell lysate transfected with a Myc-His tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 124 kDa

Observed band size: 124 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) at 1/5000 dilution

Lane 1:

HEK-293 whole cell lysate (Human epithelial cell line from embryonic kidney) transfected with a Myc-His tagged CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) vector at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

Lane 4:

Rat embryo lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 124 kDa

Observed band size: 124 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) at 1/5000 dilution

Lane 1:

HEK-293T whole cell lysate (Human epithelial cell line from embryonic kidney transfected with an empty vector containing GFP-Myc tag (vector control) at 20 µg

Lane 2:

HEK-293T whole cell lysate transfected with a GFP-Myc tagged CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) vector at 20 µg

Lane 3:

HEK-293T whole cell lysate transfected with a GFP-Myc tagged CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, aa1-800) vector at 20 µg

Lane 4:

HEK-293 whole cell lysate transfected with a GFP-Myc tagged CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus, aa801-1409) vector at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 124 kDa

Observed band size: 124 kDa

false

Exposure time: 1s

Carrier free

Anti-CRISPR-Cas9 antibody [EPR19795] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19795

Isotype

IgG

Carrier free

Reacts with

Staphylococcus aureus

Applications

WB, Flow Cyt (Intra), IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Staphylococcus aureus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Staphylococcus aureus": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

What is this antibody validated in?
Anti-CRISPR-Cas9 antibody [EPR19795] (ab203943) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Staphylococcus aureus samples.

What is the molecular weight of CRISPR-Cas9?
Anti-CRISPR-Cas9 [EPR19795] (ab203943) specifically detects a band for CRISPR-Cas9 (UniProt: J7RUA5) at a molecular weight of 124kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19795] also available for your convenience: ab203943, Carrier free - ab223153

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.

Biological function summary

The Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.

Pathways

CRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.

CRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.

See full target information CRISPR-associated endonuclease Cas9

Additional targets

CRISPR-Cas9

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 33:101536 PubMed40809677

2025

Early postnatal expression mitigates immune responses to Cas9 in the murine central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Duba-Kiss,David R Hampson

Nature communications 15:3773 PubMed38710738

2024

In vivo genome editing via CRISPR/Cas9-mediated homology-independent targeted integration for Bietti crystalline corneoretinal dystrophy treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Meng,Ruixuan Jia,Xinping Zhao,Fan Zhang,Shaohong Chen,Shicheng Yu,Xiaozhen Liu,Hongliang Dou,Xuefeng Feng,Jinlu Zhang,Ni Wang,Boling Xu,Liping Yang

eLife 12: PubMed37272616

2023

Allele-specific gene-editing approach for vision loss restoration in -associated retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhen Liu,Jing Qiao,Ruixuan Jia,Fan Zhang,Xiang Meng,Yang Li,Liping Yang

Nucleic acids research 47:9637-9657 PubMed31410472

2019

Antagonistic and synergistic epigenetic modulation using orthologous CRISPR/dCas9-based modular system.

Applications

Unspecified application

Species

Unspecified reactive species

Goran Josipović,Vanja Tadić,Marija Klasić,Vladimir Zanki,Ivona Bečeheli,Felicia Chung,Akram Ghantous,Toma Keser,Josip Madunić,Maria Bošković,Gordan Lauc,Zdenko Herceg,Aleksandar Vojta,Vlatka Zoldoš

Human gene therapy 30:571-589 PubMed30358434

2018

Somatic Gene Editing of by AAV-CRISPR/Cas9 Alters Retinal Structure and Function in Mouse and Macaque.

Applications

Unspecified application

Species

Unspecified reactive species

K Tyler McCullough,Sanford L Boye,Diego Fajardo,Kaitlyn Calabro,James J Peterson,Christianne E Strang,Dibyendu Chakraborty,Sebastian Gloskowski,Scott Haskett,Steven Samuelsson,Haiyan Jiang,C Douglas Witherspoon,Paul D Gamlin,Morgan L Maeder,Shannon E Boye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRISPR-Cas9 antibody [EPR19795]

Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

Western blot - Anti-CRISPR-Cas9 antibody [EPR19795] (AB203943)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (5)

Product promise