Rabbit Recombinant Monoclonal CRISPR-Cas9 antibody. Carrier free. Suitable for ICC, IP, WB, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Transfected cell lysate - Staphylococcus aureus, Transfected cell line - Staphylococcus aureus, Staphylococcus aureus samples.
Images
![Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunocytochemistry-img112116.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654)")
![Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunocytochemistry-immunofluorescence-img92695.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654)")
![Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunoprecipitation-img11753.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654)")
![Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunocytochemistry-img65567.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654)")
![Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--flow-cytometry-intracellular-img26811.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (AB218654)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC | IP | WB | IHC-P | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|---|---|
| Staphylococcus aureus | Predicted | Predicted | Predicted | Predicted | Predicted | Expected |
| Transfected cell line - Staphylococcus aureus | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Transfected cell lysate - Staphylococcus aureus | Tested | Tested | Expected | Not recommended | Tested | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Staphylococcus aureus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Staphylococcus aureus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Staphylococcus aureus | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Staphylococcus aureus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Staphylococcus aureus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Staphylococcus aureus | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Staphylococcus aureus | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Staphylococcus aureus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Staphylococcus aureus | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Staphylococcus aureus | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Staphylococcus aureus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Staphylococcus aureus | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Staphylococcus aureus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Staphylococcus aureus | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Staphylococcus aureus | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Staphylococcus aureus | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate - Staphylococcus aureus, Transfected cell line - Staphylococcus aureus | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
6 products for Alternative Product
Target data
Function
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.
Additional Targets
CRISPR-Cas9
Alternative names
CRISPR-associated endonuclease Cas9, SaCas9, cas9
Recommended products
Rabbit Recombinant Monoclonal CRISPR-Cas9 antibody. Carrier free. Suitable for ICC, IP, WB, IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Transfected cell lysate - Staphylococcus aureus, Transfected cell line - Staphylococcus aureus, Staphylococcus aureus samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19799
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab218654 is the carrier-free version of Anti-CRISPR-Cas9 antibody [EPR19799] ab203933.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.
- Biological function summary
The Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.
- Pathways
CRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.
- Associated diseases and disorders
CRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunocytochemistry-img112116.jpg "Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
Immunocytochemical analysis of agarose-embedded 293T (Human epithelial cell line from embryonic kidney) cells transfected with Staphylococcus aureus subsp. Aureus Cas9 (pcDNA3.1(+)-Myc-His) labeling CRISPR-Cas9 with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Positive staining on 293T cells transfected with Staphylococcus aureus subsp. Aureus Cas9 (pcDNA3.1(+)-Myc-His) is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
![Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunocytochemistry-immunofluorescence-img92695.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag or Empty vector, labeling CRISPR-Cas9 with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Confocal image showing positive staining on 293T cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
![Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunoprecipitation-img11753.jpg "Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
CRISPR-Cas9 was immunoprecipitated from 0.35 mg of HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293 whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag 10μg (Input).
Lane 2: Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 IP in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 in HEK-293 whole cell lysate transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
All lanes: Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19799] (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933)
![Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunocytochemistry-img65567.jpg "Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Immunocytochemistry - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
Immunocytochemical analysis of agarose-embedded HeLa (Human epithelial cell line from cervix adenocarcinoma) cells transfected with blank pcDNA3.1(+)-Myc-His vector labeling CRISPR-Cas9 with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.No staining on HeLa cells transfected with blank pcDNA3.1(+)-Myc-His vector.Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
![Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--flow-cytometry-intracellular-img26811.jpg "Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Flow Cytometry (Intracellular) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (Human epithelial cell line from embryonic kidney) cells transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag labeling CRISPR-Cas9 with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/60 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] -Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128030.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling CRISPR-Cas9 with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
No staining on Human endometrium is observed.
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90102.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CRISPR-Cas9 with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
No staining on mouse spleen is observed.
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116419.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CRISPR-Cas9 with Anti-CRISPR-Cas9 antibody [EPR19799] ab203933 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
No staining on rat spleen is observed.
Counter stained with Hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CRISPR-Cas9 antibody [EPR19799] ab203933).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128030.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90102.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654), expandable thumbnail](https://content.abcam.com/products/images/crispr-cas9-antibody-epr19799-bsa-and-azide-free-ab218654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116419.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19799] - BSA and Azide free (ab218654)")
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