Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free

• ICC

Supplier Data

Immunocytochemistry - Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free (AB271303)

This image was produced using ab271293, the same antibody clone but in a different formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T+OE-94 cells labelling CRISPR-Cas9 with ab271293 at 1/50 dilution (18.32 ug/ml), followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Confocal image showing cytoplasmic staining in 293T cells transfected with GFP-tagged Cas9 expression vector.
Secondary antibody only control : Secondary antibody is ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 ug/ml).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free (AB271303)

This image was produced using ab271293, the same antibody clone but in a different formulation.

Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct and (Panel B) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty plasmid, labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin.
Positive staining on (A) HEK-293T transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct, no staining on (B) HEK-293T transfected with empty plasmid.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free (AB271303)

This image was produced using ab271293, the same antibody clone but in a different formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Negative control : No staining on the human tonsil.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free (AB271303)

This image was produced using ab271293, the same antibody clone but in a different formulation.

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Negative control : No staining on the rat spleen.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free (AB271303)

This image was produced using ab271293, the same antibody clone but in a different formulation.

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
Negative control : No staining on the mouse spleen.

• WB

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free (AB271303)

This image was produced using ab271293, the same antibody clone but in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] (<a href='/en-us/products/primary-antibodies/crispr-cas9-antibody-kani345b-ab271293'>ab271293</a>) at 1/5000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate at 5 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 5 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 5 µg

Lane 4:

C6 (rat glial tumor glial cell), whole cell lysate at 5 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] - BSA and Azide free (AB271303)

This image was produced using ab271293, the same antibody clone but in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] (<a href='/en-us/products/primary-antibodies/crispr-cas9-antibody-kani345b-ab271293'>ab271293</a>) at 1/5000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate at 5 µg

Lane 2:

HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 3:

HEK-293 transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag, whole cell lysate at 5 µg

Lane 4:

HEK-293 transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus) with Myc-His tag, whole cell lysate at 5 µg

Lane 5:

HEK-293 transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag, whole cell lysate at 5 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 1s