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AB273462

Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free

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Mouse Recombinant Monoclonal Anti-CRISPR protein AcrIIA4 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Bacteriophage sp. samples.
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Western blot - Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (AB273462)
  • WB

Supplier Data

Western blot - Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] - BSA and Azide free (AB273462)

This data was produced using ab273439, the same clone in a different formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8] (<a href='/en-us/products/primary-antibodies/crispr-protein-acriia4-antibody-7c5-3a8-ab273439'>ab273439</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

HEK-293T transfected with acrIIA4 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

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Exposure time: 37s

  • Unconjugated

    Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7C5-3A8

Isotype

IgG1

Carrier free

Yes

Reacts with

Bacteriophage sp.

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Species reactivity: Bacteriophage sp.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bacteriophage sp.": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Bacteriophage sp.": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab273462 is the carrier-free version of ab273439.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Anti-CRISPR protein AcrIIA4 also known simply as AcrIIA4 acts as a potent inhibitor of the CRISPR/Cas9 system. This protein binds to the Cas9 enzyme preventing it from associating with DNA and stopping the gene-editing process. AcrIIA4 has a molecular mass of approximately 12 kDa and is expressed in various bacterial species most notably in Listeria monocytogenes. The mechanical inhibition of the CRISPR activity by AcrIIA4 is specific targeting the Cas9 DNA-binding groove to block its function effectively.
Biological function summary

This anti-CRISPR protein plays a role in bacterial defense against phages that use CRISPR/Cas systems to target bacterial cells. AcrIIA4 operates as part of a protein complex within the bacterial cell working alongside other cell machinery to ensure proper functioning of bacterial genes without interference from CRISPR systems. By blocking the CRISPR/Cas mechanism AcrIIA4 can maintain bacterial genome stability in environments where genetic modification pressure is high.

Pathways

AcrIIA4 participates in bacterial immune evasion pathways notably interfering with the CRISPR-associated immune pathway. The most important pathway related to this is the Type II CRISPR/Cas system where AcrIIA4 directly targets the Cas9 protein. This interaction prevents the cleavage of target DNA by the Cas9 complex offering insight into regulatory mechanisms that control gene-editing processes in both natural and synthetic settings. AcrIIA4 connects with other anti-CRISPR proteins which together modulate the overall impact of CRISPR activity in microbial communities.

AcrIIA4's primary implications relate to bacterial pathogenicity and potential impacts on antimicrobial resistance. Understanding AcrIIA4 in pathogens like Listeria monocytogenes helps unravel its role in microbial resistance to phage therapies which might be used to treat bacterial infections. Additionally as the inhibition mechanism can affect gene-editing efficiency AcrIIA4 is relevant in discussions about potential off-target impacts in CRISPR therapeutic applications mediated through Cas9 misregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Anti-CRISPR protein AcrIIA4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com