AB45136

Anti-Crk p38 antibody [EP242Y]

Name: Anti-Crk p38 antibody [EP242Y] (ab45136) | Abcam
Brand: Abcam Limited
SKU: AB45136
Price: 615 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal Crk p38 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK

2 Images

Lab

Western blot - Anti-Crk p38 antibody [EP242Y] (AB45136)

Western blot : Anti-Crk p38 antibody [EP242Y] ab45136 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 35 kDa in Wild-type HeLa cell lysates with no signal observed at this size in CRK knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^™ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Crk p38 antibody [EP242Y] (ab45136) at 1/1000 dilution

Lane 1:

Wild-type HeLa whole cell lysate at 20 µg

Lane 2:

Western blot - Human CRK (Crk p38) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-crk-crk-p38-knockout-hela-cell-line-ab265097'>ab265097</a>) at 20 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

K562 whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

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SDS-PAGE - Anti-Crk p38 antibody [EP242Y] (AB45136)

SDS-PAGE

Supplier Data

SDS-PAGE - Anti-Crk p38 antibody [EP242Y] (AB45136)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa cells labelling Crk with ab45136 at 1/50 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) was used as the secondary antibody.

Carrier free

Anti-Crk p38 antibody [EP242Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP242Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/50", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.. Isoform Crk-II. Regulates cell adhesion, spreading and migration (PubMed : 31311869). Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4 (PubMed : 19004829). May regulate the EFNA5-EPHA3 signaling (By similarity).

See full target information CRK

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107884 PubMed37766995

2023

Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by .

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Guo,Yuze Ma,Zhibo Jia,Liping Wang,Xinyue Lu,Yuhao Chen,Yanfeng Wang,Huifang Hao,Shuixing Yu,Zhigang Wang

Journal of ovarian research 16:79 PubMed37085900

2023

Gab2 plays a carcinogenic role in ovarian cancer by regulating CrkII.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Yin,Li Zhang,Yong Li,Can Zhang,Aiqin He

Allergologia et immunopathologia 51:146-153 PubMed36617834

2023

Chryseriol attenuates the progression of OVA-induced asthma in mice through NF-κB/HIF-1α and MAPK/STAT1 pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shangyao Mo,Hao Deng,Yong Xie,Lixia Yang,Lili Wen

Allergologia et immunopathologia 50:113-118 PubMed35527664

2022

Tricin attenuates the progression of LPS-induced severe pneumonia in bronchial epithelial cells by regulating AKT and MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Wenming Liu

Technology in cancer research & treatment 20:15330338211036523 PubMed34384283

2021

MicroRNA-215-5p Inhibits the Proliferation and Migration of Wilm's Tumor Cells by Targeting CRK.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Li,Li Lingdi,Dang Xiqiang,Liu Jiheng,Tan Xin,Huang Qin,Li Haisha

Journal of cell science 125:1814-26 PubMed22328516

2012

The metastasis gene NEDD9 product acts through integrin β3 and Src to promote mesenchymal motility and inhibit amoeboid motility.

Applications

Species

Unspecified reactive species

Jessica Ahn,Victoria Sanz-Moreno,Christopher J Marshall

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Crk p38 antibody [EP242Y]

Western blot - Anti-Crk p38 antibody [EP242Y] (AB45136)

SDS-PAGE - Anti-Crk p38 antibody [EP242Y] (AB45136)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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