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AB69723

Anti-Crk p38 antibody [mAbcam69723]

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Mouse Monoclonal Crk p38 antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

Crko, Adapter molecule crk, Proto-oncogene c-Crk, p38

1 Images
Western blot - Anti-Crk p38 antibody [mAbcam69723] (AB69723)
  • WB

Unknown

Western blot - Anti-Crk p38 antibody [mAbcam69723] (AB69723)

Crk p38 contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.

All lanes:

Western blot - Anti-Crk p38 antibody [mAbcam69723] (ab69723) at 1 µg/mL

Lane 1:

K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 4:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 5:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 33 kDa

Observed band size: 36 kDa,45 kDa

true

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam69723

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB45136

Anti-Crk p38 antibody [EP242Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Crk p38 antibody [EP242Y] (AB45136)

  • 5
  • 1
Primary Antibodies

AB76227

Anti-Crk p38 (phospho Y221) antibody [EPR269Y]

RabMAb|Recombinant

Western blot - Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (AB76227)

  • 0
  • 0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.. Isoform CRK-II : Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4 (By similarity). May regulate the EFNA5-EPHA3 signaling (PubMed : 11870224).
See full target information Crk
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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