AB76227

Anti-Crk p38 (phospho Y221) antibody [EPR269Y]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal Crk p38 phospho Y221 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK

1 Images

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Western blot - Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (AB76227)

All lanes:

Western blot - Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (ab76227) at 1/2000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

K562 cell lysate treated with AP at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR269Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects proteins phosphorylated at tyrosine 221.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.. Isoform Crk-II. Regulates cell adhesion, spreading and migration (PubMed : 31311869). Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4 (PubMed : 19004829). May regulate the EFNA5-EPHA3 signaling (By similarity).

See full target information Adapter molecule crk phospho Y221

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

World journal of diabetes 16:95092 PubMed40093279

2025

O-linked β-N-acetylglucosamine transferase regulates macrophage polarization in diabetic periodontitis: and study.

Applications

Unspecified application

Species

Unspecified reactive species

Ye-Ke Wu,Min Liu,Hong-Ling Zhou,Xiang He,Jing Wei,Wei-Han Hua,Hui-Jing Li,Qiang-Hua Yuan,Yun-Fei Xie

iScience 26:107884 PubMed37766995

2023

Crosstalk between integrin/FAK and Crk/Vps25 governs invasion of bovine mammary epithelial cells by .

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Guo,Yuze Ma,Zhibo Jia,Liping Wang,Xinyue Lu,Yuhao Chen,Yanfeng Wang,Huifang Hao,Shuixing Yu,Zhigang Wang

Oxidative medicine and cellular longevity 2022:3605977 PubMed35096267

2022

Modulating Oxidative Stress in B Cells Promotes Immunotherapy in Food Allergy.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Tao Zeng,Yu Liu,Miao Zhao,Jiang-Qi Liu,Qiao-Ruo Jin,Zhi-Qiang Liu,Yan Li,Zhi-Gang Liu,Bai-Sui Feng,Pingchang Yang

PLoS pathogens 10:e1004022 PubMed24675776

2014

Crk adaptors negatively regulate actin polymerization in pedestals formed by enteropathogenic Escherichia coli (EPEC) by binding to Tir effector.

Applications

Species

Human

Elvira Nieto-Pelegrin,Eugenia Meiler,José Manuel Martín-Villa,María Benito-León,Narcisa Martinez-Quiles

International immunopharmacology 17:785-92 PubMed24070708

2013

The p38 MAPK inhibitor JLU1124 inhibits the inflammatory response induced by lipopolysaccharide through the MAPK-NF-κB pathway in RAW264.7 macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-ning Li,Jing Su,Lu Zhao,Jing-bao Xiang,Wanhe Wang,Fei Liu,Hong-yan Li,Jia-teng Zhong,Xu Bai,Lian-kun Sun

Molecular & cellular proteomics : MCP 11:355-69 PubMed22499769

2012

Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics.

Applications

Species

Unspecified reactive species

Isabel Martinez Ferrando,Raghothama Chaerkady,Jun Zhong,Henrik Molina,Harrys K C Jacob,Katie Herbst-Robinson,Beverley M Dancy,Vikram Katju,Ron Bose,Jin Zhang,Akhilesh Pandey,Philip A Cole

Molecular cancer therapeutics 9:1419-31 PubMed20423995

2010

Effects of thymoquinone in the expression of mucin 4 in pancreatic cancer cells: implications for the development of novel cancer therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Maria P Torres,Moorthy P Ponnusamy,Subhankar Chakraborty,Lynette M Smith,Srustidhar Das,Hwyda A Arafat,Surinder K Batra

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Crk p38 (phospho Y221) antibody [EPR269Y]

Western blot - Anti-Crk p38 (phospho Y221) antibody [EPR269Y] (AB76227)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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