Rabbit Polyclonal CRKL antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CRKL aa 1-300.
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Publications
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- Cancer biotherapy & radiopharmaceuticals 35:153-1622019ZEB2-AS1 Accelerates Epithelial/Mesenchymal Transition Through miR-1205/CRKL Pathway in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinghao Jiang,Guangming Liu,Wei Ye,Jianjin Xie,Chunfa Shao,Xiaowei Wang,Xia LiPubMed 31755734
- The Journal of biological chemistry 294:16198-162132019Ligand-dependent spatiotemporal signaling profiles of the μ-opioid receptor are controlled by distinct protein-interaction networks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSrgjan Civciristov et. al.PubMed 31515267
- Oncology letters 12:3312-33222016Expression level of and combined with exon 19 deletion in and status confer differential prognosis of lung adenocarcinoma subtypes.
- Biomedical reports 2:481-4892014Differential expression of and genes in lung adenocarcinoma subtypes according to the epidermal growth factor receptor and anaplastic lymphoma kinase gene status.Applications:IHC-PReactive species:HumanDA-Ping Yu,Yu-Jie Dong,Hai-Qing Zhang,Jing-Hui Wang,Yang Qu,Li-Juan Zhou,Dan Su,Li-Li Zhang,Dan Zhao,Yi-Ran CaiPubMed 24944792
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CRKL aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P46109
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
May mediate the transduction of intracellular signals.
Alternative names
Crk-like protein, CRKL
Recommended products
Rabbit Polyclonal CRKL antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CRKL aa 1-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CRKL aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P46109
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-CrkL antibody (ab151791)
12% SDS PAGE
All lanes: Western blot - Anti-CrkL antibody (ab151791) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: H1299 whole cell lysate at 30 µg
Lane 4: HeLaS3 whole cell lysate at 30 µg
Lane 5: HepG2 whole cell lysate at 30 µg
Lane 6: Molt-4 whole cell lysate at 30 µg
Lane 7: Raji whole cell lysate at 30 µg
Predicted band size: 34 kDa
Western blot - Anti-CrkL antibody (ab151791)
12% SDS PAGE
All lanes: Western blot - Anti-CrkL antibody (ab151791) at 1/1000 dilution
All lanes: Mouse brain whole cell lysate at 50 µg
Predicted band size: 34 kDa, 41 kDa, 43 kDa, 86 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody (ab151791)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling CrkL with ab151791 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody (ab151791)
Immunohistochemical analysis of paraffin-embedded SW480 xenograft labeling CrkL with ab151791 at 1/500 dilution.
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