Anti-CrkL antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody (AB151791)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling CrkL protein at cytoplasm and nucleus using ab151791 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).
Scale bar = 10 µM

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody (AB151791)

Immunohistochemical analysis of human ovarian carcinoma tissue stained for CrkL protein at cytosol and nucleus on human ovarian carcinoma with ab151791 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody (AB151791)

Immunohistochemical analysis of human breast carcinoma tissue stained for CrkL protein at cytoplasm with ab151791 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CrkL antibody (AB151791)

Immunohistochemical analysis of human colon carcinoma tissue stained for CrkL protein at cytosol on human colon carcinoma with ab151791 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• IP

Supplier Data

Immunoprecipitation - Anti-CrkL antibody (AB151791)

Immunoprecipitation of CrkL protein from 293T whole cell extracts using 5 µg of CrkL antibody. Western blot analysis was performed using CrkL antibody.

All lanes:

Immunoprecipitation - Anti-CrkL antibody (ab151791) at 5 µg

Lane 1:

CrkL protein from 293T whole cell extracts

Lane 2:

CrkL protein from 293T whole cell extracts with Control IgG

Lane 3:

CrkL protein from 293T whole cell extracts (+)

Secondary

All lanes:

EasyBlot HRP-conjugated anti rabbit IgG antibody

Predicted band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-CrkL antibody (AB151791)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-CrkL antibody (ab151791) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Predicted band size: 34 kDa

false

• WB

Supplier Data

Western blot - Anti-CrkL antibody (AB151791)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-CrkL antibody (ab151791) at 1/1000 dilution

All lanes:

Mouse brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 34 kDa

false