Rabbit Recombinant Monoclonal CRKL antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-CrkL antibody [Y243] - BSA and Azide free (AB247234), expandable thumbnail](https://content.abcam.com/products/images/crkl-antibody-y243-bsa-and-azide-free-ab247234--western-blot-img143360.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CrkL antibody [Y243] - BSA and Azide free (AB247234)")
![Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody [Y243] - BSA and Azide free (AB247234), expandable thumbnail](https://content.abcam.com/products/images/crkl-antibody-y243-bsa-and-azide-free-ab247234--immunocytochemistry-immunofluorescence-img68170.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody [Y243] - BSA and Azide free (AB247234)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
May mediate the transduction of intracellular signals.
Alternative names
Crk-like protein, CRKL
Recommended products
Rabbit Recombinant Monoclonal CRKL antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- Y243
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247234 is the carrier-free version of Anti-CrkL antibody [Y243] ab32126.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-CrkL antibody [Y243] - BSA and Azide free (ab247234), expandable thumbnail](https://content.abcam.com/products/images/crkl-antibody-y243-bsa-and-azide-free-ab247234--western-blot-img143360.jpg "Western blot - Anti-CrkL antibody [Y243] - BSA and Azide free (ab247234)")
Western blot - Anti-CrkL antibody [Y243] - BSA and Azide free (ab247234)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CrkL antibody [Y243] ab32126).
Lanes 1-3: Merged signal (red and green). Green - Anti-CrkL antibody [Y243] ab32126 observed at 37 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-CrkL antibody [Y243] ab32126 Anti-CrkL antibody [Y243] was shown to specifically react with CrkL in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human CRKL knockout HeLa cell line ab265993 (knockout cell lysate Human CRKL knockout HeLa cell lysate ab257397) was used. Wild-type and CrkL knockout samples were subjected to SDS-PAGE. Anti-CrkL antibody [Y243] ab32126 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CrkL antibody [Y243] (Anti-CrkL antibody [Y243] ab32126) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CRKL knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human CRKL knockout HeLa cell line (Human CRKL knockout HeLa cell line ab265993)
Lane 3: K-562 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 37 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody [Y243] - BSA and Azide free (ab247234), expandable thumbnail](https://content.abcam.com/products/images/crkl-antibody-y243-bsa-and-azide-free-ab247234--immunocytochemistry-immunofluorescence-img68170.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody [Y243] - BSA and Azide free (ab247234)")
Immunocytochemistry/ Immunofluorescence - Anti-CrkL antibody [Y243] - BSA and Azide free (ab247234)
This data was developed using the same antibody clone in a different buffer formulation (Anti-CrkL antibody [Y243] ab32126).
Immunocytochemistry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) labeling CrkL with purified Anti-CrkL antibody [Y243] ab32126 at 1/100 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.6 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
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