AB52908

Anti-CrkL (phospho Y207) antibody [EP270Y]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal CRKL phospho Y207 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Crk-like protein, CRKL

1 Images

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Western blot - Anti-CrkL (phospho Y207) antibody [EP270Y] (AB52908)

All lanes:

Western blot - Anti-CrkL (phospho Y207) antibody [EP270Y] (ab52908) at 1/50000 dilution

Lane 1:

(A) K562 cell lysates untreated at 10 µg

Lane 2:

(B) K562 cell lysates treated with AP at 10 µg

Predicted band size: 34 kDa

Observed band size: 37 kDa

false

Carrier free

Anti-CrkL (phospho Y207) antibody [EP270Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP270Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May mediate the transduction of intracellular signals.

See full target information CRKL pY207

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0161748 PubMed27598410

2016

A Cell-Based Assay for Measuring Endogenous BcrAbl Kinase Activity and Inhibitor Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Steven B Ouellette,Brett M Noel,Laurie L Parker

Journal of cancer research and clinical oncology 141:283-93 PubMed25217883

2014

Inhibition of 32Dp210 cells harboring T315I mutation by a novel derivative of emodin correlates with down-regulation of BCR-ABL and its downstream signaling pathways.

Applications

Unspecified application

Species

Human

Jing Li,Yingyu Chen,Buyuan Chen,Cai Chen,Binglin Qiu,Zhihong Zheng,Jing Zheng,Tingbo Liu,Wenfeng Wang,Jianda Hu

Oncotarget 5:5257-71 PubMed24913448

2014

LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia.

Applications

Species

Unspecified reactive species

Jochen J Frietsch,Carolin Kastner,Thomas G P Grunewald,Hardy Schweigel,Peter Nollau,Janine Ziermann,Joachim H Clement,Paul La Rosée,Andreas Hochhaus,Elke Butt

Molecular oncology 7:1103-15 PubMed24012109

2013

Aberrant signalling by protein kinase CK2 in imatinib-resistant chronic myeloid leukaemia cells: biochemical evidence and therapeutic perspectives.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Borgo,Luca Cesaro,Valentina Salizzato,Maria Ruzzene,Maria Lina Massimino,Lorenzo A Pinna,Arianna Donella-Deana

PloS one 8:e56627 PubMed23437189

2013

A multiple reaction monitoring (MRM) method to detect Bcr-Abl kinase activity in CML using a peptide biosensor.

Applications

Species

Human

Tzu-Yi Yang,Christie L Eissler,Mark C Hall,Laurie L Parker

Analytical biochemistry 397:73-8 PubMed19818327

2009

A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Applications

Species

Human

Ekaterina A Placzek,Michael P Plebanek,Andrew M Lipchik,Stephanie R Kidd,Laurie L Parker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CrkL (phospho Y207) antibody [EP270Y]

Western blot - Anti-CrkL (phospho Y207) antibody [EP270Y] (AB52908)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise