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AB285090

Anti-CRLF2 antibody [EPR22662-109] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal CRLF2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CRL2, ILXR, TSLPR, CRLF2, Cytokine receptor-like factor 2, Cytokine receptor-like 2, IL-XR, Thymic stromal lymphopoietin protein receptor, TSLP receptor

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Sandwich ELISA - Anti-CRLF2 antibody [EPR22662-109] - BSA and Azide free (Detector) (AB285090)
  • sELISA

Lab

Sandwich ELISA - Anti-CRLF2 antibody [EPR22662-109] - BSA and Azide free (Detector) (AB285090)

Sandwich ELISA of ab253816 with the capture antibody ab284984 dilution at 2 µg/mL and detector antibody ab285090 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22662-109

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab285090 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CRLF2. The recommended pair for sandwich ELISA is:
Capture: ab284984, Human CRLF2 Capture Antibody (unconjugated)
Detector: ab285090, Human CRLF2 Detector Antibody (unconjugated)
The reference range value is 3.91 - 250 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRLF2 also known as cytokine receptor-like factor 2 is a receptor with a mass of approximately 40 kDa. It is primarily expressed on the cell surface of hematopoietic cells including early B-cell progenitors and certain types of leukemia cells. CRLF2 forms a part of the cytokine receptor family and its expression can be significantly increased in certain conditions most notably in some forms of leukemia.
Biological function summary

CRLF2 plays an important role in lymphoid cell development and differentiation. It partners with the interleukin-7 receptor alpha chain (IL-7Rα) to create a receptor complex. This complex is responsible for the activation of downstream signaling pathways that are key for cell survival proliferation and differentiation. The presence and regulation of this receptor complex are essential for maintaining normal hematopoietic function.

Pathways

This receptor is integral to the JAK/STAT signaling pathway. This pathway is critical for the regulation of immune cell proliferation and function. CRLF2 by binding to its ligands activates JAK kinases which then recruit and phosphorylate STAT proteins. Another important pathway associated with CRLF2 is the PI3K/AKT pathway which also gets activated upon ligand binding and plays a significant role in cell survival and growth. The IL-7Rα as part of the receptor complex is an important interacting partner in these pathways.

CRLF2 overexpression or mutation is linked to certain leukemias such as B-cell acute lymphoblastic leukemia (B-ALL). These abnormalities lead to uncontrolled proliferation and survival of leukemia cells. CRLF2 involvement in such malignancies frequently associates with other protein alterations including those in the JAK family of kinases. Aside from leukemia aberrations in this receptor have been observed in myeloproliferative disorders where similarly they aid in unchecked cell proliferation and contribute to the disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.
See full target information CRLF2
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Product promise

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For full details, please see our Terms & Conditions

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