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AB140761

Anti-CRLR antibody - Cytoplasmic domain

2

(3 Reviews)

|

(2 Publications)

Rabbit Polyclonal CRLR antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CALCRL.

View Alternative Names

CGRPR, CALCRL, Calcitonin gene-related peptide type 1 receptor, CGRP type 1 receptor, Calcitonin receptor-like receptor, CRLR

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLR antibody - Cytoplasmic domain (AB140761)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLR antibody - Cytoplasmic domain (AB140761)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon (submucosal plexus and submucosal vessel) tissue labelling CRLR with ab140761 at 34 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CALCRL. The exact immunogen used to generate this antibody is proprietary information.

Q16602

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except CALCR (56%).

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Alternative Products

Primary Antibodies

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Anti-CRLR antibody - N-terminal

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "34 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Goat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Opossum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pufferfish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Calcitonin Receptor-Like Receptor (CRLR) also known as the calcitonin receptor-like receptor functions as a G-protein-coupled receptor involved in the regulation of various physiological processes. CRLR has a molecular mass of approximately 55 kDa. This receptor is broadly expressed in numerous tissues including the cardiovascular system the central nervous system and peripheral tissues. As a receptor CRLR plays a central role in signal transduction by binding to specific peptide ligands mainly through interaction with receptor activity-modifying proteins (RAMPs) which are essential for its functional specificity.
Biological function summary

CRLR operates as part of a receptor complex with RAMPs that determines its ligand specificity; this complex enables it to bind peptides such as adrenomedullin and calcitonin gene-related peptide (CGRP). When combined with different RAMPs CRLR can switch its ligand affinity and function as adrenomedullin or CGRP receptors therefore influencing vascular tone pain transmission and metabolic regulation. Research demonstrates that CRLR and its associated complexes contribute to processes like vasodilation angiogenesis and neurogenic inflammation emphasizing its role in maintaining homeostasis.

Pathways

CRLR actively participates in critical biological pathways particularly the signaling pathways of CGRP and adrenomedullin which are significant in cardiovascular and nervous system functions. Within these pathways CRLR modulates the activity of secondary messengers such as cyclic AMP (cAMP) upon ligand binding therefore initiating further downstream effects in target cells. Related proteins including RAMPs and components of cAMP signaling work in conjunction with CRLR to ensure precise cellular responses to ligand stimulation linking it to broader physiological processes like stress response and blood pressure regulation.

CRLR stands associated with conditions such as migraines and heart failure. The ability of CRLR to bind CGRP makes it a target for migraine therapies as CGRP plays a pivotal role in migraine pathophysiology. Additionally CRLR through its regulation of adrenomedullin becomes linked to heart failure given adrenomedullin's effects on vasodilation and cardiac function. In these pathological conditions CRLR often interacts with RAMPs influencing the severity and progression of the disease capturing interest for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G protein-coupled receptor which specificity is determined by its interaction with receptor-activity-modifying proteins (RAMPs) (PubMed : 32296767, PubMed : 33602864, PubMed : 8626685). Together with RAMP1, form the receptor complex for calcitonin-gene-related peptides CALCA/CGRP1 and CALCB/CGRP2 (PubMed : 33602864). Together with RAMP2 or RAMP3, function as receptor complexes for adrenomedullin (ADM and ADM2) (PubMed : 32296767, PubMed : 9620797). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway (PubMed : 32296767, PubMed : 8626685).
See full target information CALCRL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37444550

2023

Co-Expression of Immunohistochemical Markers MRP2, CXCR4, and PD-L1 in Gallbladder Tumors Is Associated with Prolonged Patient Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Tittarelli,Omar Barría,Evy Sanders,Anna Bergqvist,Daniel Uribe Brange,Mabel Vidal,María Alejandra Gleisner,Jorge Ramón Vergara,Ignacio Niechi,Iván Flores,Cristián Pereda,Cristian Carrasco,Claudia Quezada-Monrás,Flavio Salazar-Onfray

Molecular reproduction and development 84:712-718 PubMed28621496

2017

Adrenomedullin regulates the speed of oviductal fluid flow in cattle.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Yoshimoto,Takumi Nishie,Sayaka Ito,Yoshihiko Kobayashi,Yuki Yamamoto,Kiyoshi Okuda,Koji Kimura
View all publications
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Product promise

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