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AB199722

Anti-CRMP1 antibody [EP14521]

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(5 Publications)

Rabbit Recombinant Monoclonal CRMP1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples. Cited in 5 publications.

View Alternative Names

DPYSL1, ULIP3, CRMP1, Dihydropyrimidinase-related protein 1, DRP-1, Collapsin response mediator protein 1, Inactive dihydropyrimidinase, Unc-33-like phosphoprotein 3, CRMP-1, ULIP-3

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CRMP1 with ab199722 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. No staining on Human liver tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CRMP1 antibody [EP14521] (AB199722)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CRMP1 antibody [EP14521] (AB199722)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling CRMP1 with ab199722 at 1/20 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730;black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-CRMP1 antibody [EP14521] (AB199722)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CRMP1 antibody [EP14521] (AB199722)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) and Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CRMP1 with ab199722 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and nuclear staining on U-87 MG cell line is observed. Negative expression in Jurkat cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab199722 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling CRMP1 with ab199722 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-CRMP1 antibody [EP14521] (AB199722)
  • IP

Unknown

Immunoprecipitation - Anti-CRMP1 antibody [EP14521] (AB199722)

CRMP1 was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab199722 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199722 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : Human fetal brain whole cell lysate 10ug (Input). Lane 2 : ab199722 IP in Human fetal brain whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199722 in Human fetal brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-CRMP1 antibody [EP14521] (ab199722)

Predicted band size: 62 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling CRMP1 with ab199722 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] (AB199722)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling CRMP1 with ab199722 at 1/8000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)
  • WB

Supplier Data

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)

Blocking/Dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab199722 recognizes two isoforms with the predicted MWs of 62 kDa and 74 kDa, respectively.

All lanes:

Western blot - Anti-CRMP1 antibody [EP14521] (ab199722) at 1/5000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma from bone marrow cells) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa,62 kDa

Observed band size: 52 kDa,64 kDa,80 kDa

false

Exposure time: 15s

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)
  • WB

Supplier Data

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% NFDM /TBST or 1%BSA /TBST.

Based on the sequence analysis, CRMP1 does not have similar isoforms within Mouse & Rat, therefore only a single band at approximately 62kDa is seen in Mouse and Rat lysates within western blot.

All lanes:

Western blot - Anti-CRMP1 antibody [EP14521] (ab199722) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 62 kDa

Observed band size: 64 kDa

false

Exposure time: 30s

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)
  • WB

Lab

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CRMP1 antibody [EP14521] (ab199722) at 1/1000 dilution

All lanes:

N-His tagged Recombinant Human CRMP1 protein (aa 1 to 572) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 62 kDa

Observed band size: 65 kDa

false

Exposure time: 3s

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)
  • WB

Lab

Western blot - Anti-CRMP1 antibody [EP14521] (AB199722)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CRMP1 antibody [EP14521] (ab199722) at 1/1000 dilution

All lanes:

N-GST tagged Recombinant Human CRMP2 protein (aa1-572) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 62 kDa

Observed band size: 89 kDa

false

Exposure time: 3s

  • Carrier free

    Anti-CRMP1 antibody [EP14521] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP14521

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab199722 shows no cross reactivity with CRMP2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/8000", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (PubMed : 25358863). Plays a role in axon guidance (PubMed : 25358863). During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone (PubMed : 25358863). Involved in invasive growth and cell migration (PubMed : 11562390). May participate in cytokinesis (PubMed : 19799413).
See full target information CRMP1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 29: PubMed38131196

2023

Mitochondrial abnormality in ovarian granulosa cells of patients with polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Xie,Hui Lu,Xiujia Zhang,Zhuo An,Tong Chen,Wenbo Yu,Shusong Wang,Dandan Shang,Xueying Wang

Molecular psychiatry 28:2136-2147 PubMed36973347

2023

Altered behavior, brain structure, and neurometabolites in a rat model of autism-specific maternal autoantibody exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew R Bruce,Amalie C M Couch,Simone Grant,Janna McLellan,Katherine Ku,Christina Chang,Angelica Bachman,Matthew Matson,Robert F Berman,Richard J Maddock,Douglas Rowland,Eugene Kim,Matthew D Ponzini,Danielle Harvey,Sandra L Taylor,Anthony C Vernon,Melissa D Bauman,Judy Van de Water

eNeuro 9: PubMed35523582

2022

Inhibition of Crmp1 Phosphorylation at Ser522 Ameliorates Motor Function and Neuronal Pathology in Amyotrophic Lateral Sclerosis Model Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuya Asano,Haruko Nakamura,Yuko Kawamoto,Mikiko Tada,Yayoi Kimura,Hiroshi Takano,Ryoji Yao,Hiroya Saito,Takuya Ikeda,Hiroyasu Komiya,Shun Kubota,Shunta Hashiguchi,Keita Takahashi,Misako Kunii,Kenichi Tanaka,Yoshio Goshima,Fumio Nakamura,Hideyuki Takeuchi,Hiroshi Doi,Fumiaki Tanaka

Journal of neurochemistry 157:1207-1221 PubMed33449368

2021

Phosphorylation of Collapsin Response Mediator Protein 1 (CRMP1) at Tyrosine 504 residue regulates Semaphorin 3A-induced cortical dendritic growth.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Kawashima,Aoi Jitsuki-Takahashi,Kohtaro Takizawa,Susumu Jitsuki,Takuya Takahashi,Toshio Ohshima,Yoshio Goshima,Fumio Nakamura

International journal of molecular medicine 38:1558-1564 PubMed28025999

2016

miR-200a-3p promotes the proliferation of human esophageal cancer cells by post-transcriptionally regulating cytoplasmic collapsin response mediator protein-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanzi Zang,Yong Tai,Baoluo Wan,Xiaodong Jia
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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