Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)

Rabbit Recombinant Monoclonal CRMP1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (AB251288), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Tested
Rat	Expected	Tested	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information CRMP1

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (PubMed:25358863). Plays a role in axon guidance (PubMed:25358863). During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone (PubMed:25358863). Involved in invasive growth and cell migration (PubMed:11562390). May participate in cytokinesis (PubMed:19799413).

Alternative names

DPYSL1, ULIP3, CRMP1, Dihydropyrimidinase-related protein 1, DRP-1, Collapsin response mediator protein 1, Inactive dihydropyrimidinase, Unc-33-like phosphoprotein 3, CRMP-1, ULIP-3

Recommended products

Rabbit Recombinant Monoclonal CRMP1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EP14521
Specificity: ab199722, the same antibody clone in a different buffer formulation, shows no cross reactivity with CRMP2.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab251288 is the carrier-free version of Anti-CRMP1 antibody [EP14521] ab199722.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CRMP1 antibody [EP14521] (Anti-CRMP1 antibody [EP14521] ab199722) at 1/1000 dilution
All lanes: N-GST tagged Recombinant Human CRMP2 protein (aa1-572) at 0.01 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 89 kDa
Exposure time: 3s
Western blot - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CRMP1 antibody [EP14521] (Anti-CRMP1 antibody [EP14521] ab199722) at 1/1000 dilution
All lanes: N-His tagged Recombinant Human CRMP1 protein (aa 1 to 572) at 0.01 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 65 kDa
Exposure time: 3s
Western blot - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Based on the sequence analysis, Anti-CRMP1 antibody [EP14521] ab199722 recognizes two isoforms with the predicted MWs of 62 kDa and 74 kDa, respectively.
All lanes: Western blot - Anti-CRMP1 antibody [EP14521] (Anti-CRMP1 antibody [EP14521] ab199722) at 1/5000 dilution
Lane 1: Human fetal brain lysate at 20 µg
Lane 2: SH-SY5Y (Human neuroblastoma from bone marrow cells) cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa, 62 kDa
Observed band size: 52 kDa, 64 kDa, 80 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) and Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CRMP1 with Anti-CRMP1 antibody [EP14521] ab199722 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm and nuclear staining on U-87 MG cell line is observed. Negative expression in Jurkat cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-
-ve control 1: Anti-CRMP1 antibody [EP14521] ab199722 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Western blot - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% NFDM /TBST or 1% BSA/TBST.
Based on the sequence analysis, CRMP1 does not have similar isoforms within mouse and rat, therefore only a single band at approximately 62kDa is seen in mouse and rat lysates within western blot.
All lanes: Western blot - Anti-CRMP1 antibody [EP14521] (Anti-CRMP1 antibody [EP14521] ab199722) at 1/1000 dilution
Lane 1: Mouse brain lysate at 10 µg
Lane 2: C6 (Rat glial tumor cells) cell lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 64 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling CRMP1 with Anti-CRMP1 antibody [EP14521] ab199722 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm and nucleus staining on Human cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling CRMP1 with Anti-CRMP1 antibody [EP14521] ab199722 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on mouse cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling CRMP1 with Anti-CRMP1 antibody [EP14521] ab199722 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasm staining on rat cerebral cortex is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.CRMP1 was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with Anti-CRMP1 antibody [EP14521] ab199722 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-CRMP1 antibody [EP14521] ab199722 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Lane 1: Human fetal brain whole cell lysate 10ug (Input). Lane 2: Anti-CRMP1 antibody [EP14521] ab199722 IP in Human fetal brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CRMP1 antibody [EP14521] ab199722 in Human fetal brain whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 3 seconds.
All lanes: Immunoprecipitation - Anti-CRMP1 antibody [EP14521] (Anti-CRMP1 antibody [EP14521] ab199722)
Predicted band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CRMP1 with Anti-CRMP1 antibody [EP14521] ab199722 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. No staining on Human liver tissue is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-CRMP1 antibody [EP14521] - BSA and Azide free (ab251288)
This data was developed using Anti-CRMP1 antibody [EP14521] ab199722, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling CRMP1 with Anti-CRMP1 antibody [EP14521] ab199722 at 1/20 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.