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AB250215

Anti-CRMP1 antibody [EPR14520(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal CRMP1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

DPYSL1, ULIP3, CRMP1, Dihydropyrimidinase-related protein 1, DRP-1, Collapsin response mediator protein 1, Inactive dihydropyrimidinase, Unc-33-like phosphoprotein 3, CRMP-1, ULIP-3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - BSA and Azide free (AB250215)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - BSA and Azide free (AB250215)

This data was developed using ab180517, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CRMP1 with ab180517 at 1/500 dilution. Anti-rabbit HRP seconday antibody was used and counterstaining with Hematoxylin was performed.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-CRMP1 antibody [EPR14520(B)] - BSA and Azide free (AB250215)
  • WB

Supplier Data

Western blot - Anti-CRMP1 antibody [EPR14520(B)] - BSA and Azide free (AB250215)

This data was developed using ab180517, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/crmp1-antibody-epr14520b-n-terminal-ab180517'>ab180517</a>) at 1/20000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

Fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 62 kDa

false

Western blot - Anti-CRMP1 antibody [EPR14520(B)] - BSA and Azide free (AB250215)
  • WB

Supplier Data

Western blot - Anti-CRMP1 antibody [EPR14520(B)] - BSA and Azide free (AB250215)

This data was developed using ab180517, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/crmp1-antibody-epr14520b-n-terminal-ab180517'>ab180517</a>) at 1/5000 dilution

All lanes:

Human cerebellum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 40 kDa,52 kDa,62 kDa

Observed band size: 47 kDa

false

  • Unconjugated

    Anti-CRMP1 antibody [EPR14520(B)] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14520(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Anti-STIP1/STI1 antibody [EPR6605]

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Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

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Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

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Primary Antibodies

AB129082

Anti-CRMP1 + CRMP2 antibody [EPR7792]

RabMAb|Recombinant|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB129082)

  • 0
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250215 is the carrier-free version of ab180517.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (PubMed : 25358863). Plays a role in axon guidance (PubMed : 25358863). During the axon guidance process, acts downstream of SEMA3A to promote FLNA dissociation from F-actin which results in the rearrangement of the actin cytoskeleton and the collapse of the growth cone (PubMed : 25358863). Involved in invasive growth and cell migration (PubMed : 11562390). May participate in cytokinesis (PubMed : 19799413).
See full target information CRMP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com