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Rabbit Recombinant Monoclonal CRMP2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 21 publications.


Images

Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB129082), expandable thumbnail
  • Immunoprecipitation - Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB129082), expandable thumbnail
  • Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB129082), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB129082), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB129082), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Expected
Tested
Mouse
Expected
Expected
Tested
Tested
Tested
Rat
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/100 - 1/250.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10000 - 1/50000

Notes

-

Species

Rat

Dilution info

1/10000 - 1/50000

Notes

-

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50 - 1/100

Notes

-

Expected
Expected

Species

Human, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10 - 1/1000

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Human

Dilution info

1/10 - 1/1000

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

Targets

Dihydropyrimidinase-related protein 1

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CRMP2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 21 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR7792

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Dissociation constant

2.38 x 10-10 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    All lanes: Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082) at 1/10000 dilution

    Lane 1: PC12 cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: U87 MG cell lysate at 10 µg

    Lane 4: NIH 3T3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Observed band size: 64 kDa

  • Immunoprecipitation - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Immunoprecipitation - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    ab129082 (purified) at 1:20 dilution (0.5μg) immunoprecipitating CRMP2 in U-87 MG whole cell lysate.
    Lane 1 (input): U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab129082 & U-87 MG whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab129082 in U-87 MG whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    Predicted band size: 47 kDa

  • Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    Blocking and dilution buffer: 5% NFDM /TBST

    All lanes: Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082) at 1/1000 dilution

    Lanes 1 and 3: Western blot - Recombinant Human CRMP1 protein (Recombinant Human CRMP1 protein ab202599)

    Lanes 2 and 4: Western blot - Recombinant Human CRMP2 protein (Recombinant Human CRMP2 protein ab114269)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 3s

    THis data was developed using ab129082, the same antibody clone in a diffrent buffer formulation.

    Blocking and dilution buffer: 5% NFDM /TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CRMP2 with Purified ab129082 at 1:1000 dilution (0.12 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling CRMP2 with Purified ab129082 at 1:1000 dilution (0.12 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates

    All lanes: Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082) at 1/50000 dilution

    Lane 1: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 64 kDa

  • Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    All lanes: Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082) at 1/100000 dilution

    Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 64 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    ab129082 staining CRMP2 in NIH/3T3 (mouse embryonic fibroblast) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • Flow Cytometry (Intracellular) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    Overlay histogram showing SH-SY5Y cells stained with ab129082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129082, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    ab129082, at a dilution of 1/100, staining CRMP2 in paraffin embedded Human astrocytoma tissue by Immunohistochemistry.
    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    OI-RD Scanning - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Flow Cytometry (Intracellular) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CRMP1 + CRMP2 antibody [EPR7792] (ab129082)

    Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling CRMP2 with Purified ab129082 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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