Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

ab129082, at a dilution of 1/100, staining CRMP2 in paraffin embedded Human astrocytoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling CRMP2 with Purified ab129082 at 1 : 1000 dilution (0.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CRMP2 with Purified ab129082 at 1 : 1000 dilution (0.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

Overlay histogram showing SH-SY5Y cells stained with ab129082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129082, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1 µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

• IP

Unknown

Immunoprecipitation - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

ab129082 (purified) at 1 : 20 dilution (0.5μg) immunoprecipitating CRMP2 in U-87 MG whole cell lysate.
Lane 1 (input) : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab129082 & U-87 MG whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129082 in U-87 MG whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

All lanes:

Immunoprecipitation - Anti-CRMP1 + CRMP2 antibody [EPR7792] (<a href='/en-us/products/primary-antibodies/crmp1-crmp2-antibody-epr7792-ab129082'>ab129082</a>)

Predicted band size: 47 kDa

false

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

Intracellular Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling CRMP2 with Purified ab129082 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

ab129082 staining CRMP2 in NIH/3T3 (mouse embryonic fibroblast) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Negative control 1 : PBS only.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129082)

• WB

Lab

Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

THis data was developed using ab129082, the same antibody clone in a diffrent buffer formulation.

Blocking and dilution buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-CRMP1 + CRMP2 antibody [EPR7792] (<a href='/en-us/products/primary-antibodies/crmp1-crmp2-antibody-epr7792-ab129082'>ab129082</a>) at 1/1000 dilution

Lanes 1 and 3:

Western blot - Recombinant Human CRMP1 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-crmp1-protein-ab202599'>ab202599</a>)

Lanes 2 and 4:

Western blot - Recombinant Human CRMP2 protein (GST tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-crmp2-protein-ab114269'>ab114269</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 3s

• OI-RD Scanning

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OI-RD Scanning - Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free (AB240952)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.