Anti-CRMP2 antibody

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CRMP2 antibody (AB36201)

ab36201 staining human CRMP2 in olfactory receptor neurons within the olfactory mucosa by immunohistochemistry.

• WB

Lab

Western blot - Anti-CRMP2 antibody (AB36201)

Western blot : Anti-DPYSL2 antibody (ab36201) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab36201 was shown to bind specifically to DPYSL2. A band was observed at 63 kDa in wild-type A549 cell lysates with no signal observed at this size in DPYSL2 knockout cell line. To generate this image, wild-type and DPYSL2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CRMP2 antibody (ab36201) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

DPYSL2 knockout A549 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

A549 Nuclear Fraction cell lysate at 20 µg

Lane 5:

Recombinant Human CRMP2 protein (<a href='/en-us/products/proteins-peptides/recombinant-human-crmp2-protein-ab114269'>ab114269</a>) cell lysate at 0.2 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 63 kDa

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• WB

Unknown

Western blot - Anti-CRMP2 antibody (AB36201)

All lanes:

Western blot - Anti-CRMP2 antibody (ab36201)

Lane 1:

E.coli transformed with cDNA of human CRMP1

Lane 2:

E.coli transformed with cDNA of human CRMP2

Lane 3:

E.coli transformed with cDNA of human CRMP3

Lane 4:

E.coli transformed with cDNA of human CRMP4

Lane 5:

E.coli transformed with cDNA of human CRMP5

Predicted band size: 62 kDa

Observed band size: 65 kDa

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