Rabbit Polyclonal CRMP2 antibody. Suitable for WB, IHC-Fr and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human DPYSL2.
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- Cell death & disease 9:2972018Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna P Simões et. al.PubMed 29463792
- Scientific reports 7:75952017Whole transcriptome profiling of taste bud cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunil K Sukumaran et. al.PubMed 28790351
- Journal of neurochemistry 122:775-882012Synaptic cell adhesion molecule-2 and collapsin response mediator protein-2 are novel members of the matrix metalloproteinase-9 degradome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalgorzata Bajor et. al.PubMed 22694054
- Journal of neurochemistry 118:616-252011The memory enhancing effect of the APP-derived tripeptide Ac-rER is mediated through CRMP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRadmila Mileusnic et. al.PubMed 21255016
- Hippocampus 21:108-192009Proteomics reveal rat hippocampal lateral asymmetry.Applications:WBReactive species:RatA Samara,K Vougas,A Papadopoulou,E Anastasiadou,N Baloyanni,E Paronis,G P Chrousos,G Th TsangarisPubMed 20020437
- Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 5:92-1022009Synaptic proteins linked to HIV-1 infection and immunoproteasome induction: proteomic analysis of human synaptosomes.
- Biochemical and biophysical research communication 381:393-62009Regulation of ROCKII by localization to membrane compartments and binding to DynaminI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSylvester Tumusiime et. al.PubMed 19222995
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DPYSL2. The exact immunogen used to generate this antibody is proprietary information. Database link Q16555
Reactivity data
Select an application| WB | IHC-Fr | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
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Target data
Function
Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.
Alternative names
CRMP2, ULIP2, DPYSL2, Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, Unc-33-like phosphoprotein 2, CRMP-2, ULIP-2
Recommended products
Rabbit Polyclonal CRMP2 antibody. Suitable for WB, IHC-Fr and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human DPYSL2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DPYSL2. The exact immunogen used to generate this antibody is proprietary information. Database link Q16555
- Purity
- Whole antiserum
- Specificity
No cross reactivity was detected with CRMP1, CRMP3, CRMP4 or CRMP5.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-CRMP2 antibody (ab36201)
All lanes: Western blot - Anti-CRMP2 antibody (ab36201)
Lane 1: E.coli transformed with cDNA of human CRMP1
Lane 2: E.coli transformed with cDNA of human CRMP2
Lane 3: E.coli transformed with cDNA of human CRMP3
Lane 4: E.coli transformed with cDNA of human CRMP4
Lane 5: E.coli transformed with cDNA of human CRMP5
Predicted band size: 62 kDa
Observed band size: 65 kDa
Immunohistochemistry (Frozen sections) - Anti-CRMP2 antibody (ab36201)
ab36201 staining human CRMP2 in olfactory receptor neurons within the olfactory mucosa by immunohistochemistry.
Western blot - Anti-CRMP2 antibody (ab36201)
Western blot: Anti-DPYSL2 antibody (ab36201) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab36201 was shown to bind specifically to DPYSL2. A band was observed at 63 kDa in wild-type A549 cell lysates with no signal observed at this size in DPYSL2 knockout cell line. To generate this image, wild-type and DPYSL2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CRMP2 antibody (ab36201) at 1/2000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: DPYSL2 knockout A549 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: A549 Nuclear Fraction cell lysate at 20 µg
Lane 5: Recombinant Human CRMP2 protein (Recombinant Human CRMP2 protein ab114269) cell lysate at 0.2 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 63 kDa
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