Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)

Knockout Tested Rabbit Recombinant Monoclonal CRMP2 antibody. Carrier free. Suitable for WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Western blot - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (AB315286), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IP	ICC/IF	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Tested	Expected
Rat	Tested	Expected	Tested	Tested	Expected
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information Dihydropyrimidinase-related protein 2

Function

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

Alternative names

CRMP2, ULIP2, DPYSL2, Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, Unc-33-like phosphoprotein 2, CRMP-2, ULIP-2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28480-66
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab315286 is the carrier-free version of Anti-CRMP2 antibody [EPR28480-66] ab315285.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Western blot - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using the same antibody clone in a different buffer formulation (abAB315285).
Western blot: Anti-DPYSL2 antibody [EPR28480-66] (Anti-CRMP2 antibody [EPR28480-66] ab315285) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-CRMP2 antibody [EPR28480-66] ab315285 was shown to bind specifically to DPYSL2. A band was observed at 63 kDa in wild-type A549 cell lysates with no signal observed at this size in DPYSL2 knockout cell line. To generate this image, wild-type and DPYSL2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CRMP2 antibody [EPR28480-66] (Anti-CRMP2 antibody [EPR28480-66] ab315285) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: DPYSL2 knockout A549 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: A549 Nuclear Fraction cell lysate at 20 µg
Lane 5: Recombinant Human CRMP2 protein (Recombinant Human CRMP2 protein ab114269) cell lysate at 0.2 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 63 kDa
Western blot - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-CRMP2 antibody [EPR28480-66] (Anti-CRMP2 antibody [EPR28480-66] ab315285) at 1/1000 dilution
Lane 1: His-tagged human DPYSL1 protein at 10 ng
Lane 2: His-tagged human DPYSL2 protein at 10 ng
Lane 3: His-tagged human DPYSL3 protein at 10 ng
Lane 4: His-tagged human DPYSL4 protein at 10 ng
Lane 5: His-tagged human DPYSL5 protein at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 62 kDa
Exposure time: 37s
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/1000 (0.503 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/1000 (0.503 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/1000 (0.503 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in SH-SY5Y cell line.Low or negative expression: SK-BR-3 (PMID: 32778769). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat kidney. The section was incubated with Anti-CRMP2 antibody [EPR28480-66] ab315285 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse kidney. The section was incubated with Anti-CRMP2 antibody [EPR28480-66] ab315285 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining on rat cerebrum. The section was incubated with Anti-CRMP2 antibody [EPR28480-66] ab315285 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining on mouse cerebrum. The section was incubated with Anti-CRMP2 antibody [EPR28480-66] ab315285 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining on human cerebrum. The section was incubated with Anti-CRMP2 antibody [EPR28480-66] ab315285 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CRMP2 antibody [EPR28480-66] (Anti-CRMP2 antibody [EPR28480-66] ab315285) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 20 µg
Lane 2: Human hypothalamus tissue lysate at 20 µg
Lane 3: Mouse hippocampus tissue lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Lane 5: Rat hippocampus tissue lysate at 20 µg
Lane 6: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 62 kDa, 36 kDa
Exposure time: 1s
Western blot - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low or negative expression: K-562, SK-BR-3 (PMID: 32778769).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-CRMP2 antibody [EPR28480-66] (Anti-CRMP2 antibody [EPR28480-66] ab315285) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 2: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 7: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 62 kDa, 36 kDa
Exposure time: 10s
Flow Cytometry (Intracellular) - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SK-BR-3 (human breast adenocarcinoma epithelial cell, Left) / SH-SY5Y (human neuroblastoma epithelial cell, Right) cells labelling CRMP2 with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/5000 dilution (0.01 ug, Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Low or negative expression: SK-BR-3 (PMID: 32778769).
Immunoprecipitation - Anti-CRMP2 antibody [EPR28480-66] - BSA and Azide free (ab315286)
This data was developed using Anti-CRMP2 antibody [EPR28480-66] ab315285, the same antibody clone in a different buffer formulation.
CRMP2 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CRMP2 antibody [EPR28480-66] ab315285 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2: Anti-CRMP2 antibody [EPR28480-66] ab315285 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CRMP2 antibody [EPR28480-66] ab315285 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-CRMP2 antibody [EPR28480-66] (Anti-CRMP2 antibody [EPR28480-66] ab315285) at 1/30 dilution
All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 3s