Rabbit Recombinant Monoclonal CRMP3 antibody. Suitable for WB and reacts with Mouse, Human, Recombinant fragment - Human samples. Cited in 1 publication.
Images
![Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (AB128875), expandable thumbnail](https://content.abcam.com/products/images/crmp2-crmp3-crmp4-antibody-epr7590-ab128875--western-blot-img116358.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (AB128875)")
![Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (AB128875), expandable thumbnail](https://content.abcam.com/products/images/crmp2-crmp3-crmp4-antibody-epr7590-ab128875--western-blot-img433175.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (AB128875)")
Publications
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- American journal of cancer research 12:713-7282022Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Hsuan Hung,Ming-Derg Lai,Wen-Chun Hung,Li-Tzong ChenPubMed 35261797
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Predicted | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/50000 | Notes - |
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species Recombinant fragment - Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).
Additional Targets
DPYSL3, DPYSL2
Alternative names
CRMP3, ULIP4, DPYSL4, Dihydropyrimidinase-related protein 4, DRP-4, Collapsin response mediator protein 3, UNC33-like phosphoprotein 4, CRMP-3, ULIP-4
Recommended products
Rabbit Recombinant Monoclonal CRMP3 antibody. Suitable for WB and reacts with Mouse, Human, Recombinant fragment - Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7590
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875), expandable thumbnail](https://content.abcam.com/products/images/crmp2-crmp3-crmp4-antibody-epr7590-ab128875--western-blot-img116358.jpg "Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875)")
Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875)
All lanes: Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875) at 1/10000 dilution
Lane 1: SH-SYSY cell lysate at 10 µg
Lane 2: Neuro-2a cell lysate at 10 µg
Lane 3: U-87 MG cell lysate at 10 µg
Lane 4: Human cerebellum lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa
![Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875), expandable thumbnail](https://content.abcam.com/products/images/crmp2-crmp3-crmp4-antibody-epr7590-ab128875--western-blot-img433175.jpg "Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875)")
Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as a loading control at 1/10000 dilution.
All lanes: Western blot - Anti-CRMP2 + CRMP3 + CRMP4 antibody [EPR7590] (ab128875) at 1/10000 dilution
Lane 1: His tagged full length CRMP1 recombinant protein at 15 ng
Lane 2: His tagged full length CRMP3 recombinant protein at 15 ng
Lane 3: His tagged full length CRMP2 recombinant protein at 15 ng
Lane 4: His tagged full length CRMP4 recombinant protein at 15 ng
Lane 5: His tagged full length CRMP5 recombinant protein at 15 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50-75 kDa
Exposure time: 80s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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