AB62478

Anti-CRMP2 (phospho T514) antibody

Name: Anti-CRMP2 (phospho T514) antibody (ab62478) | Abcam
Brand: Abcam Limited
SKU: AB62478
Price: 545 USD
Availability: InStock

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(16 Publications)

Rabbit Polyclonal CRMP2 phospho T514 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human DPYSL2 phospho T514 aa 450-550.

View Alternative Names

CRMP2, ULIP2, DPYSL2, Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, Unc-33-like phosphoprotein 2, CRMP-2, ULIP-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 (phospho T514) antibody (AB62478)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 (phospho T514) antibody (AB62478)

ab62478, at 1/50 dilution, staining CRMP2 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (AB62478)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (AB62478)

ab62478, at 1/500 dilution, staining CRMP2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (AB62478)

Immunofluorescence analysis of RPE (human retinal pigmented epithelium) cells, staining CRMP2 (phospho T514) with ab62478. Cells were fixed in cold methanol and incubated with primary antibody, followed by an AlexaFluor®488-conjugated anti rabbit IgG.

Image from Ou Y et al., PLoS ONE 7(11): e48773. Fig 5.; doi: 10.1371/journal.pone.0048773. Epub 2012 Nov 21.

Unknown

Western blot - Anti-CRMP2 (phospho T514) antibody (AB62478)

All lanes:

Western blot - Anti-CRMP2 (phospho T514) antibody (ab62478) at 1 µg/mL

All lanes:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 62 kDa

Observed band size: 63 kDa,70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

WB, ICC/IF, ELISA, IHC-P

applications

Immunogen

Synthetic Peptide within Human DPYSL2 phospho T514 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q16555

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab62478 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

See full target information DPYSL2 phospho T514

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 9:32 PubMed38351062

2024

Docking protein 6 (DOK6) selectively docks the neurotrophic signaling transduction to restrain peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Guo,Pan Xiang,Xiaojiao Sun,Wei Liu,Jiafeng Zhou,Bin Yin,Lin Hou,Boqin Qiang,Huiliang Li,Pengcheng Shu,Xiaozhong Peng

Frontiers in pharmacology 12:763181 PubMed34955834

2021

Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Maxim. Treatment in a Rat Model of Focal Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Le Yang,Jian-Feng Lei,Jun-Yao Ouyang,Man-Zhong Li,Yu Zhan,Xue-Feng Feng,Yun Lu,Ming-Cong Li,Lei Wang,Hai-Yan Zou,Hui Zhao

International journal of molecular sciences 22: PubMed34638704

2021

Nogo-A Induced Polymerization of Microtubule Is Involved in the Inflammatory Heat Hyperalgesia in Rat Dorsal Root Ganglion Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Chen,Qiguo Hu,Huaicun Liu,Yan Zhao,Sun-On Chan,Jun Wang

Nature medicine 27:954-963 PubMed34083813

2021

Biomarkers for neurodegenerative diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Oskar Hansson

Biomedicines 9: PubMed33401710

2021

Somatostatin Ameliorates β-Amyloid-Induced Cytotoxicity via the Regulation of CRMP2 Phosphorylation and Calcium Homeostasis in SH-SY5Y Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seungil Paik,Rishi K Somvanshi,Helen A Oliveira,Shenglong Zou,Ujendra Kumar

Acta pharmacologica Sinica 41:1557-1567 PubMed32265492

2020

Downregulation of hippocampal SIRT6 activates AKT/CRMP2 signaling and ameliorates chronic stress-induced depression-like behavior in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Li,Xin Liu,Hui Qiao

Neuropharmacology 158:107712 PubMed31325432

2019

Role of serotonin 4 receptor in the growth of hippocampal neurons during the embryonic development in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lokesh Agrawal,Sunil Kumar Vimal,Takashi Shiga

Advances in neurobiology 15:491-528 PubMed28674995

2017

Biomarkers in Neurodegenerative Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Jeromin,Robert Bowser

Journal of neurochemistry 137:446-59 PubMed26788931

2016

Conventional protein kinase Cβ-mediated phosphorylation inhibits collapsin response-mediated protein 2 proteolysis and alleviates ischemic injury in cultured cortical neurons and ischemic stroke-induced mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Yang,Xinxin Zhang,Yun Li,Song Han,David W Howells,Shujuan Li,Junfa Li

Nature communications 6:6366 PubMed25721514

2015

Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis.

Applications

Unspecified application

Species

Unspecified reactive species

Elise Arbeille,Florie Reynaud,Isabelle Sanyas,Muriel Bozon,Karine Kindbeiter,Frédéric Causeret,Alessandra Pierani,Julien Falk,Frédéric Moret,Valérie Castellani

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRMP2 (phospho T514) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 (phospho T514) antibody (AB62478)

Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (AB62478)

Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (AB62478)

Western blot - Anti-CRMP2 (phospho T514) antibody (AB62478)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (16)

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