AB129066

Anti-CRMP2 (phospho T514) antibody [EPR7876]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal CRMP2 phospho T514 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

CRMP2, ULIP2, DPYSL2, Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, Unc-33-like phosphoprotein 2, CRMP-2, ULIP-2

2 Images

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Western blot - Anti-CRMP2 (phospho T514) antibody [EPR7876] (AB129066)

The lower panel was analysed using a monoclonal antibody raised against non-phosphoryated CRMP2.

All lanes:

Western blot - Anti-CRMP2 (phospho T514) antibody [EPR7876] (ab129066) at 1/1000 dilution

Lane 1:

Human fetal brain lysate, untreated at 10 µg

Lane 2:

Human fetal brain lysate, treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 62 kDa

false

OI-RD Scanning - Anti-CRMP2 (phospho T514) antibody [EPR7876] (AB129066)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CRMP2 (phospho T514) antibody [EPR7876] (AB129066)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7876

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

See full target information DPYSL2 phospho T514

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36293516

2022

Treadmill Exercise Reduces Neuroinflammation, Glial Cell Activation and Improves Synaptic Transmission in the Prefrontal Cortex in 3 × Tg-AD Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lianwei Mu,Dongdong Xia,Jiajia Cai,Boya Gu,Xiaojie Liu,Vladislav Friedman,Qing-Song Liu,Li Zhao

Frontiers in molecular neuroscience 10:190 PubMed28663724

2017

Activation of Transient Receptor Potential Vanilloid 4 Impairs the Dendritic Arborization of Newborn Neurons in the Hippocampal Dentate Gyrus through the AMPK and Akt Signaling Pathways.

Applications

Species

Mouse

Yujing Tian,Mengwen Qi,Zhouqing Wang,Chunfeng Wu,Zhen Sun,Yingchun Li,Sha Sha,Yimei Du,Lei Chen,Ling Chen

Nature communications 7: PubMed27249678

2016

Brain-specific Crmp2 deletion leads to neuronal development deficits and behavioural impairments in mice.

Applications

Species

Mouse

Hongsheng Zhang,Eunchai Kang,Yaqing Wang,Chaojuan Yang,Hui Yu,Qin Wang,Zheyu Chen,Chen Zhang,Kimberly M Christian,Hongjun Song,Guo-Li Ming,Zhiheng Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRMP2 (phospho T514) antibody [EPR7876]

Western blot - Anti-CRMP2 (phospho T514) antibody [EPR7876] (AB129066)

OI-RD Scanning - Anti-CRMP2 (phospho T514) antibody [EPR7876] (AB129066)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

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