Anti-CRMP3 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP3 antibody (AB36217)

IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Unknown

Western blot - Anti-CRMP3 antibody (AB36217)

All lanes:

Western blot - Anti-CRMP3 antibody (ab36217)

Lane 1:

E.coli transformed with cDNA of human CRMP1

Lane 2:

E.coli transformed with cDNA of human CRMP2

Lane 3:

E.coli transformed with cDNA of human CRMP3

Lane 4:

E.coli transformed with cDNA of human CRMP4

Lane 5:

E.coli transformed with cDNA of human CRMP5

Predicted band size: 62 kDa

Observed band size: 65 kDa

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