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AB244319

Anti-CRMP4 antibody

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(2 Publications)

Rabbit Polyclonal CRMP4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DPYSL3 aa 100-250.

View Alternative Names

CRMP4, DRP3, ULIP, ULIP1, DPYSL3, Dihydropyrimidinase-related protein 3, DRP-3, Collapsin response mediator protein 4, Unc-33-like phosphoprotein 1, CRMP-4, ULIP-1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CRMP4 antibody (AB244319)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CRMP4 antibody (AB244319)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling CRMP4 using ab244319 at 4 μg/ml (green) in ICC/IF.

Western blot - Anti-CRMP4 antibody (AB244319)
  • WB

Unknown

Western blot - Anti-CRMP4 antibody (AB244319)

All lanes:

Western blot - Anti-CRMP4 antibody (ab244319) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 62 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP4 antibody (AB244319)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP4 antibody (AB244319)

Formalin-fixed, paraffin-embedded human smooth muscle tissue stained for CRMP4 with ab244319 at a 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-CRMP4 antibody (AB244319)
  • WB

Unknown

Western blot - Anti-CRMP4 antibody (AB244319)

All lanes:

Western blot - Anti-CRMP4 antibody (ab244319) at 0.4 µg/mL

All lanes:

NTERA-2 cl.D1 [NT2/D1] (Human malignant pluripotent embryonic carcinoma cell line) cell lysate

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DPYSL3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q14195

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity).
See full target information DPYSL3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 16:5829-5845 PubMed39544779

2024

Zhi Zhu Ma Ren pill relieves constipation in mice through endoplasmic reticulum stress-mediated apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wen,Yu Zhan,Jun Li,Ling Xu,Chengzi Huang,Rong Wu,Xuegui Tang

Journal of integrative neuroscience 20:595-603 PubMed34645092

2021

The impact of CRMP4 SUMOylation on the Cav1.2 interaction, neurite outgrowth and thermal pain sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Shangdao Lai,Meiyuan Pan,Hongxing Liao,Jiayu Chen,Yuqing Jiang,Yalan Li
View all publications
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Product promise

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