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AB175450

Anti-CROT/COT antibody

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(4 Publications)

Goat Polyclonal CROT/COT antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Rat Crot aa 150-200.

View Alternative Names

COT, CROT, Peroxisomal carnitine O-octanoyltransferase

1 Images
Western blot - Anti-CROT/COT antibody (AB175450)
  • WB

Supplier Data

Western blot - Anti-CROT/COT antibody (AB175450)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-CROT/COT antibody (ab175450) at 1 µg/mL

All lanes:

mouse liver lysates at 35 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Rat Crot aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P11466

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab175450 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester.
See full target information CROT

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 53:112-122 PubMed39786883

2025

Acanthoside B attenuates NLRP3-mediated pyroptosis and ulcerative colitis through inhibition of tAGE/RAGE pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo He,Chunfang Zhou,Rui Shang,Xiaoyan Wang

Heliyon 9:e18134 PubMed37539197

2023

Proteomics reveals defective peroxisomal fatty acid oxidation during the progression of acute kidney injury and repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Chen,Quan-You Zheng,Li-Ming Wang,Jia Luo,Ke-Hong Chen,Ya-Ni He

Cell death & disease 12:111 PubMed33479191

2021

Mesenchymal stem cells ameliorate lipid metabolism through reducing mitochondrial damage of hepatocytes in the treatment of post-hepatectomy liver failure.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Lin Wang,Hao-Ran Ding,Chen-Yan Pan,Xiao-Lei Shi,Hao-Zhen Ren

Bioscience reports : PubMed32373939

2020

LncRNA EGOT regulates the proliferation and apoptosis of colorectal cancer by miR-33b-5p/CROT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Ni,Chunbo Li,Chen Bo,Bomiao Zhang,Yanlong Liu,Xuefeng Bai,Bingbing Cui,Peng Han
View all publications

Product promise

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