AB72141

Anti-CRSP2 / MED14 antibody

Name: Anti-CRSP2 / MED14 antibody (ab72141) | Abcam
Brand: Abcam Limited
SKU: AB72141
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal CRSP2 / MED14 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170, MED14, Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, Cofactor required for Sp1 transcriptional activation subunit 2, Mediator complex subunit 14, RGR1 homolog, Thyroid hormone receptor-associated protein complex 170 kDa component, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, CRSP complex subunit 2, hRGR1, Trap170

2 Images

Unknown

Immunoprecipitation - Anti-CRSP2 / MED14 antibody (AB72141)

Immunoprecipitation/ Western Blot of CRSP2 / MED14.
Lane 1 : ab72141 at 3μg/mg whole cell lysate for IP.
Lane 2 : Control IgG.

ab72141 at 1μg/ml for subsequent WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-CRSP2 / MED14 antibody (ab72141)

Predicted band size: 161 kDa

false

Unknown

Western blot - Anti-CRSP2 / MED14 antibody (AB72141)

Various other bands are detected.

All lanes:

Western blot - Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Lane 3:

Whole cell lysate from Hela cells at 5 µg

Predicted band size: 161 kDa

Observed band size: 120 kDa,160 kDa,71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

See full target information MED14

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell genomics 3:100250 PubMed36950384

2023

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Greta Pintacuda,Yu-Han H Hsu,Kalliopi Tsafou,Ka Wan Li,Jacqueline M Martín,Jackson Riseman,Julia C Biagini,Joshua K T Ching,Daya Mena,Miguel A Gonzalez-Lozano,Shawn B Egri,Jake Jaffe,August B Smit,Nadine Fornelos,Kevin C Eggan,Kasper Lage

Communications biology 4:414 PubMed33772081

2021

MED15 prion-like domain forms a coiled-coil responsible for its amyloid conversion and propagation.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Batlle,Isabel Calvo,Valentin Iglesias,Cian J Lynch,Marcos Gil-Garcia,Manuel Serrano,Salvador Ventura

The Journal of biological chemistry 289:27665-76 PubMed25100719

2014

Characterization of the influence of mediator complex in HIV-1 transcription.

Applications

Unspecified application

Species

Human

Alba Ruiz,Eduardo Pauls,Roger Badia,Eva Riveira-Muñoz,Bonaventura Clotet,Ester Ballana,José A Esté

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRSP2 / MED14 antibody

Immunoprecipitation - Anti-CRSP2 / MED14 antibody (AB72141)

Western blot - Anti-CRSP2 / MED14 antibody (AB72141)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise