AB192261

Anti-CRTAP/CASP antibody [EPR15121] - N-terminal

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CRTAP/CASP antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CASP, CRTAP, Cartilage-associated protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (AB192261)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (AB192261)

Immunohistochemical analysis of paraffin embedded human colon adenocarcinoma tissue sections labeling CRTAP/CASP using ab192261 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (AB192261)

Immunohistochemical analysis of paraffin embedded human endometrial tissue sections labeling CRTAP/CASP using ab192261 at a 1/250 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (AB192261)

All lanes:

Western blot - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (ab192261) at 1/1000 dilution

Lane 1:

Saos-2 cell lysate at 20 µg

Lane 2:

SW1353 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

false

Carrier free

Anti-CRTAP/CASP antibody [EPR15121] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15121

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues.

See full target information CRTAP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bone research 12:10 PubMed38378678

2024

RANKL inhibition reduces lesional cellularity and Gα variant expression and enables osteogenic maturation in fibrous dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Luis F de Castro,Jarred M Whitlock,Zachary Michel,Kristen Pan,Jocelyn Taylor,Vivian Szymczuk,Brendan Boyce,Daniel Martin,Vardit Kram,Rebeca Galisteo,Kamran Melikov,Leonid V Chernomordik,Michael T Collins,Alison M Boyce

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CRTAP/CASP antibody [EPR15121] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (AB192261)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (AB192261)

Western blot - Anti-CRTAP/CASP antibody [EPR15121] - N-terminal (AB192261)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise