Anti-CRTAP/CASP antibody [EPR15122(B)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15122(B)] - BSA and Azide free (AB250694)

This data was developed using ab183720, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human trachea tissue sections labeling CRTAP/CASP using ab183720 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTAP/CASP antibody [EPR15122(B)] - BSA and Azide free (AB250694)

This data was developed using ab183720, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue sections labeling CRTAP/CASP using ab183720 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-CRTAP/CASP antibody [EPR15122(B)] - BSA and Azide free (AB250694)

This data was developed using ab183720, the same antibody clone in a different buffer formulation.

Lysate from SW1353 cells was immunoprecipitated with ab183720 at a 1/50 dilution. For the subsequent blot, ab183720 was used at a 1/1000 dilution with Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Lane 2 : TBS instead of SW1353 lysates. Blocking/ Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CRTAP/CASP antibody [EPR15122(B)] (<a href='/en-us/products/primary-antibodies/crtap-casp-antibody-epr15122b-ab183720'>ab183720</a>)

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] - BSA and Azide free (AB250694)

This data was developed using ab183720, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] (<a href='/en-us/products/primary-antibodies/crtap-casp-antibody-epr15122b-ab183720'>ab183720</a>) at 1/5000 dilution

All lanes:

A431 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] - BSA and Azide free (AB250694)

This data was developed using ab183720, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRTAP/CASP antibody [EPR15122(B)] (<a href='/en-us/products/primary-antibodies/crtap-casp-antibody-epr15122b-ab183720'>ab183720</a>) at 1/20000 dilution

Lane 1:

Saos-2 whole cell lysate at 20 µg

Lane 2:

SW1353 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 47 kDa

false