Rabbit Recombinant Monoclonal CRTC3 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 12 publications.
Images
![Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--western-blot-img154122.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118465.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106823.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104331.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)")
![Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--western-blot-img83942.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)")
Publications
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- Cell death discovery 9:3312023In vitro CRISPR screening uncovers CRTC3 as a regulator of IFN-γ-induced ferroptosis of hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Li,Tao Xing,Yiran Chen,Weiran Xu,Bo Fan,Gaoda Ju,Jing Zhao,Li Lin,Cihui Yan,Jun Liang,Xiubao RenPubMed 37666810
- Journal of nanobiotechnology 20:4532022TiSe-mediated sonodynamic and checkpoint blockade combined immunotherapy in hypoxic pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLibin Chen,Wang Xue,Jing Cao,Shengmin Zhang,Yiqing Zeng,Ling Ma,Xuechen Qian,Qing Wen,Yurong Hong,Zhan Shi,Youfeng XuPubMed 36243711
- Arteriosclerosis, thrombosis, and vascular biology 41:2431-24512021Salt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujun Cai,Xue-Lin Wang,Jinny Lu,Xin Lin,Jonathan Dong,Raul J GuzmanPubMed 34196217
- Reproduction (Cambridge, England) 161:573-5802021TORC2/3-mediated DUSP1 upregulation is essential for human decidualization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunfang Xu,Weijie Zhao,Xixi Huang,Zhuxuan Jiang,Lu Liu,Liyuan Cui,Xinyi Li,Dajin Li,Meirong DuPubMed 33780908
- Journal of neuroendocrinology 33:e129202020Activation and expression of endogenous CREB-regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorna I F Smith,Zidong Zhao,Jamie Walker,Stafford Lightman,Francesca SpigaPubMed 33314405
- Molecular and cellular endocrinology 499:1106122019Involvement of CREB-regulated transcription coactivators (CRTC) in transcriptional activation of steroidogenic acute regulatory protein (Star) by ACTH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorna I F Smith,Victoria Huang,Mark Olah,Loc Trinh,Ying Liu,Georgina Hazell,Becky Conway-Campbell,Zidong Zhao,Antoine Martinez,Anne-Marie Lefrançois-Martinez,Stafford Lightman,Francesca Spiga,Greti AguileraPubMed 31604124
- Cancer discovery 9:1606-16272019The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive Effects of LKB1 in NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPablo E Hollstein et. al.PubMed 31350328
- Journal of leukocyte biology 99:711-212015SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Stella Lombardi,Corine Gilliéron,Damien Dietrich,Cem GabayPubMed 26590148
- The Journal of neuroscience : the official journal of the Society for Neuroscience 35:12248-602015Oxytocin Regulates Stress-Induced Crf Gene Transcription through CREB-Regulated Transcription Coactivator 3.Applications:ChIP, WBReactive species:HumanBenjamin Jurek,David A Slattery,Yuichi Hiraoka,Ying Liu,Katsuhiko Nishimori,Greti Aguilera,Inga D Neumann,Erwin H van den BurgPubMed 26338335
- Nature communications 5:45352014The LKB1-salt-inducible kinase pathway functions as a key gluconeogenic suppressor in the liver.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Expected | Not recommended | Tested | Tested |
| Rat | Expected | Not recommended | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20000 | Notes For unpurified, use 1/1000 - 10000. |
Species Human | Dilution info 1/20000 | Notes For unpurified, use 1/1000 - 10000. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
Alternative names
TORC3, CRTC3, CREB-regulated transcription coactivator 3, Transducer of regulated cAMP response element-binding protein 3, TORC-3, Transducer of CREB protein 3
Recommended products
Rabbit Recombinant Monoclonal CRTC3 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3440
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--western-blot-img154122.jpg "Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654)")
Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution
Lane 1: Raw 264.7 whole cell lysate at 20 µg
Lane 2: PC-12 whole cell lysate at 20 µg
Lane 3: NIH/3T3 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 76 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118465.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)
Immunohistochemical staining of paraffin embedded rat spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106823.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)
Immunohistochemical staining of paraffin embedded mouse spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104331.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)
Immunohistochemical staining of paraffin embedded human tonsil with purified ab91654 at a working dilution of 1/250. The secondary antibody used is Goat Anti-Rabbit IgG H&L (HRP) ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--western-blot-img83942.jpg "Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654)")
Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution
Lane 1: HEK293 whole cell lysate at 20 µg
Lane 2: HeLa whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 76 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121626.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)
Unpurified ab91654 at 1/100 dilution staining CRTC3 in Human tonsil by immunohistochemistry (paraffin-embedded section).
![Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--western-blot-img129489.jpg "Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654)")
Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654)
All lanes: Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/1000 dilution
Lane 1: 293T cell lysates at 10 µg
Lane 2: Jurkat cell lysates at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 67 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121626.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (ab91654)")
![Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654), expandable thumbnail](https://content.abcam.com/products/images/crtc3-antibody-epr3440-ab91654--western-blot-img129489.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654)")
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