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AB91654

Anti-CRTC3 antibody [EPR3440]

5

(2 Reviews)

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(15 Publications)

Anti-CRTC3 antibody [EPR3440] (ab91654) is a rabbit monoclonal antibody detecting CRTC3 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2010

View Alternative Names

TORC3, CRTC3, CREB-regulated transcription coactivator 3, Transducer of regulated cAMP response element-binding protein 3, TORC-3, Transducer of CREB protein 3

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Unpurified ab91654 at 1/100 dilution staining CRTC3 in Human tonsil by immunohistochemistry (paraffin-embedded section).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Immunohistochemical staining of paraffin embedded mouse spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRTC3 antibody [EPR3440] (AB91654)

Immunohistochemical staining of paraffin embedded rat spleen with purified ab91654 at a working dilution of 1/250. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • WB

Lab

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution

Lane 1:

HEK293 whole cell lysate at 20 µg

Lane 2:

HeLa whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

Observed band size: 76 kDa

false

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • WB

Unknown

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)

All lanes:

Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/1000 dilution

Lane 1:

293T cell lysates at 10 µg

Lane 2:

Jurkat cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 67 kDa

false

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • WB

Lab

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

CRTC3 appears to undergo degradation (PMID : 21164481).

All lanes:

Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/10000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate. (Fresh) at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate. (Fresh) at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate. (Frozen) at 20 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate. (Frozen) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 76 kDa

false

Exposure time: 180s

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)
  • WB

Lab

Western blot - Anti-CRTC3 antibody [EPR3440] (AB91654)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-CRTC3 antibody [EPR3440] (ab91654) at 1/20000 dilution

Lane 1:

Raw 264.7 whole cell lysate at 20 µg

Lane 2:

PC-12 whole cell lysate at 20 µg

Lane 3:

NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 67 kDa

Observed band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3440

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p>CRTC3 is susceptible to degradation (PMID: 21164481). It is strongly recommended to use freshly prepared lysates for Western blot analysis.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-CRTC3 antibody [EPR3440] (ab91654) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of CRTC3?
Anti-CRTC3 [EPR3440] (ab91654) specifically detects a band for CRTC3 (UniProt: Q6UUV7) at a molecular weight of 67kDa.

Trusted by the scientific community
Anti-CRTC3 [EPR3440] (ab91654) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [EPR3440] also available for your convenience: ab91654, Alexa Fluor® 488 - ab206248, Carrier free - ab220809, Biotin - ab302836

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).
See full target information CRTC3
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 15:69-83 PubMed39949945

2025

Triptolide induces immunogenic cell death in cervical cancer cells via ER stress and redox modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwen Zheng,Yamei Chen,Chao Wang,Ling Li,Buzhen Tan

Journal of medicinal chemistry 67:5233-5258 PubMed38552030

2024

Discovery of Clinical Candidate GLPG3970: A Potent and Selective Dual SIK2/SIK3 Inhibitor for the Treatment of Autoimmune and Inflammatory Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Christophe Peixoto,Agnes Joncour,Taouès Temal-Laib,Amynata Tirera,Aurélie Dos Santos,Hélène Jary,Denis Bucher,Wendy Laenen,Anna Pereira Fernandes,Stephanie Lavazais,Carole Delachaume,Didier Merciris,Corinne Saccomani,Michael Drennan,Miriam López-Ramos,Emanuelle Wakselman,Sonia Dupont,Monica Borgonovi,Carlos Roca Magadan,Alain Monjardet,Reginald Brys,Steve De Vos,Martin Andrews,Juan-Miguel Jimenez,David Amantini,Nicolas Desroy

Cell death discovery 9:331 PubMed37666810

2023

In vitro CRISPR screening uncovers CRTC3 as a regulator of IFN-γ-induced ferroptosis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Tao Xing,Yiran Chen,Weiran Xu,Bo Fan,Gaoda Ju,Jing Zhao,Li Lin,Cihui Yan,Jun Liang,Xiubao Ren

Journal of nanobiotechnology 20:453 PubMed36243711

2022

TiSe-mediated sonodynamic and checkpoint blockade combined immunotherapy in hypoxic pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Libin Chen,Wang Xue,Jing Cao,Shengmin Zhang,Yiqing Zeng,Ling Ma,Xuechen Qian,Qing Wen,Yurong Hong,Zhan Shi,Youfeng Xu

Arteriosclerosis, thrombosis, and vascular biology 41:2431-2451 PubMed34196217

2021

Salt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yujun Cai,Xue-Lin Wang,Jinny Lu,Xin Lin,Jonathan Dong,Raul J Guzman

Reproduction (Cambridge, England) 161:573-580 PubMed33780908

2021

TORC2/3-mediated DUSP1 upregulation is essential for human decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Chunfang Xu,Weijie Zhao,Xixi Huang,Zhuxuan Jiang,Lu Liu,Liyuan Cui,Xinyi Li,Dajin Li,Meirong Du

Journal of neuroendocrinology 33:e12920 PubMed33314405

2020

Activation and expression of endogenous CREB-regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna I F Smith,Zidong Zhao,Jamie Walker,Stafford Lightman,Francesca Spiga

Cell death & disease 11:49 PubMed31969556

2020

Salt-inducible kinases (SIKs) regulate TGFβ-mediated transcriptional and apoptotic responses.

Applications

Unspecified application

Species

Unspecified reactive species

Luke D Hutchinson,Nicola J Darling,Stephanos Nicolaou,Ilaria Gori,Daniel R Squair,Philip Cohen,Caroline S Hill,Gopal P Sapkota

Molecular and cellular endocrinology 499:110612 PubMed31604124

2019

Involvement of CREB-regulated transcription coactivators (CRTC) in transcriptional activation of steroidogenic acute regulatory protein (Star) by ACTH.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna I F Smith,Victoria Huang,Mark Olah,Loc Trinh,Ying Liu,Georgina Hazell,Becky Conway-Campbell,Zidong Zhao,Antoine Martinez,Anne-Marie Lefrançois-Martinez,Stafford Lightman,Francesca Spiga,Greti Aguilera

Cancer discovery 9:1606-1627 PubMed31350328

2019

The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive Effects of LKB1 in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo E Hollstein,Lillian J Eichner,Sonja N Brun,Anwesh Kamireddy,Robert U Svensson,Liliana I Vera,Debbie S Ross,T J Rymoff,Amanda Hutchins,Hector M Galvez,April E Williams,Maxim N Shokhirev,Robert A Screaton,Rebecca Berdeaux,Reuben J Shaw
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com