Rabbit Recombinant Monoclonal CRX antibody. Suitable for IHC-P, WB, mIHC and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-CRX antibody [EPR9582] (AB140603), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-ab140603--western-blot-img78028.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRX antibody [EPR9582] (AB140603)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] (AB140603), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-ab140603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65658.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] (AB140603)")
![Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (AB140603), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-ab140603--multiplex-immunohistochemistry-img422538.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (AB140603)")
Publications
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- Pediatric blood & cancer 71:e307172023Immunohistochemical expression of TFF1 is a marker of poor prognosis in retinoblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRosario Aschero,Daiana Ganiewich,Gabriela Lamas,Camilo A Restrepo-Perdomo,Daniela Ottaviani,Santiago Zugbi,Sandra Camarero,Ezequiel Néspoli,Maria Cuadrado Vilanova,Sara Perez-Jaume,Guillem Pascual-Pasto,Claudia Sampor,Nathalia Grigorovski,Beatriz Salas,Mariona Suñol,Angel M Carcaboso,Jaume Mora,María T G de Dávila,François Doz,François Radvanyi,David H Abramson,Andrea S Llera,Paula S Schaiquevich,Fabiana Lubieniecki,Guillermo L ChantadaPubMed 37814421
- Molecular therapy : the journal of the American Society of Gene Therapy 31:825-8462023Photoreceptor laminin drives differentiation of human pluripotent stem cells to photoreceptor progenitors that partially restore retina function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwee Goon Tay,Helder Andre,Vicki Chrysostomou,Swarnaseetha Adusumalli,Jing Guo,Xiaoyuan Ren,Wei Sheng Tan,Jia En Tor,Aida Moreno-Moral,Flavia Plastino,Hammurabi Bartuma,Zuhua Cai,Sai Bo Bo Tun,Veluchamy Amutha Barathi,Gavin Tan Siew Wei,Gianluca Grenci,Li Yen Chong,Arne Holmgren,Anders Kvanta,Jonathan Guy Crowston,Enrico Petretto,Karl TryggvasonPubMed 36638800
- Nature communications 12:55782021A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Liu,Daniela Ottaviani,Meriem Sefta,Céline Desbrousses,Elodie Chapeaublanc,Rosario Aschero,Nanor Sirab,Fabiana Lubieniecki,Gabriela Lamas,Laurie Tonon,Catherine Dehainault,Clément Hua,Paul Fréneaux,Sacha Reichman,Narjesse Karboul,Anne Biton,Liliana Mirabal-Ortega,Magalie Larcher,Céline Brulard,Sandrine Arrufat,André Nicolas,Nabila Elarouci,Tatiana Popova,Fariba Némati,Didier Decaudin,David Gentien,Sylvain Baulande,Odette Mariani,Florent Dufour,Sylvain Guibert,Céline Vallot,Livia Lumbroso-Le Rouic,Alexandre Matet,Laurence Desjardins,Guillem Pascual-Pasto,Mariona Suñol,Jaume Catala-Mora,Genoveva Correa Llano,Jérôme Couturier,Emmanuel Barillot,Paula Schaiquevich,Marion Gauthier-Villars,Dominique Stoppa-Lyonnet,Lisa Golmard,Claude Houdayer,Hervé Brisse,Isabelle Bernard-Pierrot,Eric Letouzé,Alain Viari,Simon Saule,Xavier Sastre-Garau,François Doz,Angel M Carcaboso,Nathalie Cassoux,Celio Pouponnot,Olivier Goureau,Guillermo Chantada,Aurélien de Reyniès,Isabelle Aerts,François RadvanyiPubMed 34552068
- International journal of molecular sciences 22:2021Tumor Necrosis Factor-Alpha Exacerbates Viral Entry in SARS-CoV2-Infected iPSC-Derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiu-Yang Lee,Chih-Heng Huang,Elham Rastegari,Vimalan Rengganaten,Ping-Cheng Liu,Ping-Hsing Tsai,Yuan-Fan Chin,Jing-Rong Wu,Shih-Hwa Chiou,Yuan-Chi Teng,Chih-Wei Lee,Yanwen Liang,An-Yu Chen,Shu-Chen Hsu,Yi-Jen Hung,Jun-Ren Sun,Chian-Shiu Chien,Yueh ChienPubMed 34576032
- International journal of molecular sciences 22:2021Expression of Endogenous Angiotensin-Converting Enzyme 2 in Human Induced Pluripotent Stem Cell-Derived Retinal Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenkie Isahwan Ahmad Mulyadi Lai et. al.PubMed 33525682
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | mIHC | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, such as NRL, RORB and RAX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors.
Alternative names
CORD2, CRX, Cone-rod homeobox protein
Recommended products
Rabbit Recombinant Monoclonal CRX antibody. Suitable for IHC-P, WB, mIHC and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9582
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CRX antibody [EPR9582] (ab140603), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-ab140603--western-blot-img78028.jpg "Western blot - Anti-CRX antibody [EPR9582] (ab140603)")
Western blot - Anti-CRX antibody [EPR9582] (ab140603)
All lanes: Western blot - Anti-CRX antibody [EPR9582] (ab140603) at 1/1000 dilution
All lanes: Y79 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] (ab140603), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-ab140603--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65658.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] (ab140603)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] (ab140603)
Immunohistochemical analysis of paraffin-embedded human retina tissue labeling CRX with ab140603 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (ab140603), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-ab140603--multiplex-immunohistochemistry-img422538.jpg "Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (ab140603)")
Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (ab140603)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human retina tissue labeling PAX6, Glutamine Synthetase and CRX with Anti-PAX6 antibody [EPR3352(2)] ab109233 at 1/10000 dilution, Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free ab240193 at 1/20000 dilution and Anti-CRX antibody [EPR9582] - BSA and Azide free ab248897 at 1/1000 dilution followed by a ready to use Opal Polymer HRP Ms + Rb secondary antibody. Nuclear counter stain used was DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Panel A: merged staining of anti-CRX (gray; Opal™690), anti-Glutamine Synthetase (green; Opal™520) and anti-PAX6 (red; Opal™570) on human retina.
Panel B: anti-PAX6 stained on retinal progenitor cells.
Panel C: anti-Glutamine Synthetase stained on Müller glia.
Panel D: anti-CRX stained on subset cells of outer nuclear layer and inner nuclear layer.
The section was incubated in three rounds of staining: in the order of Anti-CRX antibody [EPR9582] - BSA and Azide free ab248897, Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free ab240193, and Anti-PAX6 antibody [EPR3352(2)] ab109233 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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