Anti-CRX antibody [EPR9582]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] (AB140603)

Immunohistochemical analysis of paraffin-embedded human retina tissue labeling CRX with ab140603 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (AB140603)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human retina tissue labeling PAX6, Glutamine Synthetase and CRX with ab109233 at 1/10000 dilution, ab240193 at 1/20000 dilution and ab248897 at 1/1000 dilution followed by a ready to use Opal Polymer HRP Ms + Rb secondary antibody. Nuclear counter stain used was DAPI. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins Panel A : merged staining of anti-CRX (gray; Opal™690), anti-Glutamine Synthetase (green; Opal™520) and anti-PAX6 (red; Opal™570) on human retina. Panel B : anti-PAX6 stained on retinal progenitor cells. Panel C : anti-Glutamine Synthetase stained on Müller glia. Panel D : anti-CRX stained on subset cells of outer nuclear layer and inner nuclear layer. The section was incubated in three rounds of staining : in the order of ab248897, ab240193, and ab109233 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (AB140603)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse retina tissue staining RBPMS with ab309094 at a 1/2000 dilution, ab140603 anti-CPX used at 1/2000 dilution and ab259971 anti-GAT1 used at a 1/500 dilution.

Panel A : merged staining of anti-RBPMS (green; Opal™520), anti-CPX (magenta; Opal™690) and anti-GAT1 (gray; Opal™570) on mouse retina.

Panel B : anti-RBPMS staining ganglion cell layer in mouse retina.

Panel C : ant-CPX staining outer nuclear layer and inner nuclear layer in mouse retina.

Panel D : ant-GAT1 staining inner plexiform layer in mouse retina.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab309094, ab140603 and ab259971 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (AB140603)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse retina tissue staining RPE65 with ab231782 at a 1/8000 dilution, ab140603 anti-CPX used at 1/2000 dilution and ab221664 anti-RHO used at a 1/30000 dilution.

Panel A : merged staining of anti-RPE65 (green; Opal™520), anti-CPX (magenta; Opal™690) and anti-RHO (gray; Opal™570) on mouse retina.

Panel B : anti-RPE65 staining pigmented layer in mouse retina.

Panel C : ant-CPX staining outer nuclear layer and inner nuclear layer in mouse retina.

Panel D : ant-RHO staining rod-shaped photoreceptor cells in mouse retina.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab231782, ab140603 and ab221664 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] (AB140603)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat retina tissue staining RBPMS with ab309094 at a 1/2000 dilution, ab140603 anti-CPX used at 1/2000 dilution and ab259971 anti-GAT1 used at a 1/500 dilution.

Panel A : merged staining of anti-RBPMS (green; Opal™520), anti-CPX (magenta; Opal™690) and anti-GAT1 (gray; Opal™570) on rat retina.

Panel B : anti-RBPMS staining ganglion cell layer in rat retina.

Panel C : ant-CPX staining outer nuclear layer and inner nuclear layer in rat retina.

Panel D : ant-GAT1 staining inner plexiform layer in rat retina.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab309094, ab140603 and ab259971 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Unknown

Western blot - Anti-CRX antibody [EPR9582] (AB140603)

All lanes:

Western blot - Anti-CRX antibody [EPR9582] (ab140603) at 1/1000 dilution

All lanes:

Y79 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 32 kDa

Observed band size: 37 kDa

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