Rabbit Recombinant Monoclonal CRX antibody. Carrier free. Suitable for IHC-P, WB, mIHC and reacts with Human samples.
Images
![Western blot - Anti-CRX antibody [EPR9582] - BSA and Azide free (AB248897), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-bsa-and-azide-free-ab248897--western-blot-img78028.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CRX antibody [EPR9582] - BSA and Azide free (AB248897)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] - BSA and Azide free (AB248897), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-bsa-and-azide-free-ab248897--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65658.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] - BSA and Azide free (AB248897)")
![Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] - BSA and Azide free (AB248897), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-bsa-and-azide-free-ab248897--multiplex-immunohistochemistry-img422538.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] - BSA and Azide free (AB248897)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | mIHC | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, such as NRL, RORB and RAX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors.
Alternative names
CORD2, CRX, Cone-rod homeobox protein
Recommended products
Rabbit Recombinant Monoclonal CRX antibody. Carrier free. Suitable for IHC-P, WB, mIHC and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9582
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248897 is the carrier-free version of Anti-CRX antibody [EPR9582] ab140603.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-bsa-and-azide-free-ab248897--western-blot-img78028.jpg "Western blot - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897)")
Western blot - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897)
This data was developed using Anti-CRX antibody [EPR9582] ab140603, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CRX antibody [EPR9582] (Anti-CRX antibody [EPR9582] ab140603) at 1/1000 dilution
All lanes: Y79 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 37 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-bsa-and-azide-free-ab248897--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65658.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897)
This data was developed using Anti-CRX antibody [EPR9582] ab140603, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human retina tissue labeling CRX with Anti-CRX antibody [EPR9582] ab140603 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP). Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897), expandable thumbnail](https://content.abcam.com/products/images/crx-antibody-epr9582-bsa-and-azide-free-ab248897--multiplex-immunohistochemistry-img422538.jpg "Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897)")
Multiplex immunohistochemistry - Anti-CRX antibody [EPR9582] - BSA and Azide free (ab248897)
This data was developed using Anti-CRX antibody [EPR9582] ab140603, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Human retina tissue labeling PAX6, Glutamine Synthetase and CRX with Anti-PAX6 antibody [EPR3352(2)] ab109233 at 1/10000 dilution, Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free ab240193 at 1/20000 dilution and ab248897 at 1/1000 dilution followed by a ready to use Opal Polymer HRP Ms + Rb secondary antibody. Nuclear counter stain used was DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Panel A: merged staining of anti-CRX (gray; Opal™690), anti-Glutamine Synthetase (green; Opal™520) and anti-PAX6 (red; Opal™570) on human retina.
Panel B: anti-PAX6 stained on retinal progenitor cells.
Panel C: anti-Glutamine Synthetase stained on Müller glia.
Panel D: anti-CRX stained on subset cells of outer nuclear layer and inner nuclear layer.
The section was incubated in three rounds of staining: in the order of ab248897, Anti-Glutamine Synthetase antibody [EPR13022(B)] - BSA and Azide free ab240193, and Anti-PAX6 antibody [EPR3352(2)] ab109233 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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