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AB252971

Anti-CRYBB2 antibody

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(2 Publications)

Rabbit Polyclonal CRYBB2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CRYBB2 aa 100-150.

View Alternative Names

CRYB2, CRYB2A, CRYBB2, Beta-crystallin B2, Beta-B2 crystallin, Beta-crystallin Bp

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)

Paraffin-embedded, formalin-fixed human cerebral cortex tissue shows absence of immunoreactivity for CRYBB2 when incubated with ab252971 at 1/200 dilution (negative control).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)

Paraffin-embedded, formalin-fixed human testis tissue shows absence of immunoreactivity for CRYBB2 when incubated with ab252971 at 1/200 dilution (negative control).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)

Paraffin-embedded, formalin-fixed human pancreas tissue shows absence of immunoreactivity for CRYBB2 when incubated with ab252971 at 1/200 dilution (negative control).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRYBB2 antibody (AB252971)

Paraffin-embedded human eye tissue stained for CRYBB2 using ab252971 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CRYBB2 antibody (AB252971)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CRYBB2 antibody (AB252971)

PFA-fixed, Triton X-100 permeabilized SH-SY5Y (Human osteosarcoma cell line) cells cells stained for CRYBB2 (green) using ab252971 at 4 µg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CRYBB2 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P43320

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Crystallins are the dominant structural components of the vertebrate eye lens.
See full target information CRYBB2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6876 PubMed40715139

2025

The mechanotransducer Piezo1 coordinates metabolism and inflammation to promote skin growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchao Xue,Elizabeth Winnicki,Zhaoxu Zhang,Ines Lopez,Saifeng Wang,Charles Kirby,Sam S Lee,Ang Li,Chaewon Lee,Hana Minsky,Kaitlin Williams,Kevin Yueh-Hsun Yang,Ling He,Sashank K Reddy,Luis A Garza

Experimental and therapeutic medicine 24:539 PubMed35837031

2022

Formation of three-dimensional cell aggregates expressing lens-specific proteins in various cultures of human iris-derived tissue cells and iPS cells.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Hiramatsu,Naoki Yamamoto,Yu Kato,Noriaki Nagai,Sumito Isogai,Kazuyoshi Imaizumi
View all publications
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Product promise

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