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AB96089

Anti-CSB antibody

4

(1 Review)

|

(14 Publications)

Rabbit Polyclonal CSB antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 14 publications.

View Alternative Names

CSB, ERCC6, DNA excision repair protein ERCC-6, ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-CSB antibody (AB96089)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CSB antibody (AB96089)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling CSB protein at nucleus using ab96089 at a 1/500 dilution (Green).
phalloidin, a cytoskeleton marker, was stained at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)

Immunohistochemical analysis of rat brain tissue stained for CSB protein at nucleus with ab96089 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)

Immunohistochemical analysis of mouse muscle tissue stained for CSB protein at nucleus with ab96089 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)

Immunohistochemical analysis of rat hind brain tissue stained for CSB protein at nucleus with ab96089 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSB antibody (AB96089)

Immunohistochemical analysis of mouse brain tissue stained for CSB protein at nucleus with ab96089 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Mouse, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.4% Tris, 1.5% Glycine, 0.04% EGTA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed : 16246722, PubMed : 20541997, PubMed : 26620705). Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA (PubMed : 15548521). Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function (PubMed : 25820262). Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle (PubMed : 25820262). Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing premature entry of cells into mitosis following the induction of DNA DSBs (PubMed : 25820262). Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function. Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR (PubMed : 29203878). Required for stable recruitment of ELOA and CUL5 to DNA damage sites (PubMed : 28292928). Involved in UV-induced translocation of ERCC8 to the nuclear matrix (PubMed : 26620705). Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis (PubMed : 24874740).
See full target information ERCC6
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40744501

2025

Genome-wide mapping of formaldehyde-induced DNA-protein crosslinks reveals unique patterns of formation and transcription-coupled removal in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Duha Alshareef,Charlie T Nguyen,Kayla N Tucker,Micah D Gearhart,Natalia Y Tretyakova,Colin Campbell

Cell death and differentiation : PubMed40374849

2025

Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Komal Pekhale,Vinod Tiwari,Mansoor Hussain,Christy C Bridges,Deborah L Croteau,Moshe Levi,Avi Z Rosenberg,Briana Santo,Xiaoping Yang,Tomasz Kulikowicz,Xiaoxin X Wang,Jong-Hyuk Lee,Vilhelm A Bohr

Cell discovery 9:107 PubMed37907457

2023

Heat shock protein DNAJA2 regulates transcription-coupled repair by triggering CSB degradation via chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Huang,Liya Gu,Guo-Min Li

Nature communications 13:974 PubMed35190564

2022

Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Namrata Kumar,Arjan F Theil,Vera Roginskaya,Yasmin Ali,Michael Calderon,Simon C Watkins,Ryan P Barnes,Patricia L Opresko,Alex Pines,Hannes Lans,Wim Vermeulen,Bennett Van Houten

Journal of the American Chemical Society 143:20988-21002 PubMed34855372

2021

Cockayne Syndrome B Protein Selectively Resolves and Interact with Intermolecular DNA G-Quadruplex Structures.

Applications

Unspecified application

Species

Unspecified reactive species

Denise Liano,Souroprobho Chowdhury,Marco Di Antonio

Genes 12: PubMed34946871

2021

Identification and Characterization of a Novel Recurrent Variant in Patients with a Severe Form of Cockayne Syndrome B.

Applications

Unspecified application

Species

Unspecified reactive species

Khouloud Zayoud,Ichraf Kraoua,Asma Chikhaoui,Nadège Calmels,Sami Bouchoucha,Cathy Obringer,Clément Crochemore,Dorra Najjar,Sinda Zarrouk,Najoua Miladi,Vincent Laugel,Miria Ricchetti,Ilhem Turki,Houda Yacoub-Youssef

Frontiers in cell and developmental biology 9:727836 PubMed34746125

2021

Functions of the CSB Protein at Topoisomerase 2 Inhibitors-Induced DNA Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Franciele Faccio Busatto,Sofiane Y Mersaoui,Yilun Sun,Yves Pommier,Jean-Yves Masson,Jenifer Saffi

Molecular cell 80:1013-1024.e6 PubMed33338401

2020

A Surge of DNA Damage Links Transcriptional Reprogramming and Hematopoietic Deficit in Fanconi Anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Shen,Rui Wang,Moon Jong Kim,Qianghua Hu,Chih-Chao Hsu,Jun Yao,Naeh Klages-Mundt,Yanyan Tian,Erica Lynn,Thomas F Brewer,Yilei Zhang,Banu Arun,Boyi Gan,Michael Andreeff,Shunichi Takeda,Junjie Chen,Jae-Il Park,Xiaobing Shi,Christopher J Chang,Sung Yun Jung,Jun Qin,Lei Li

PLoS genetics 16:e1008555 PubMed32271760

2020

XPF-ERCC1 protects liver, kidney and blood homeostasis outside the canonical excision repair pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Lee Mulderrig,Juan I Garaycoechea

Nature communications 10:5576 PubMed31811121

2019

CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Clément Crochemore,Cristina Fernández-Molina,Benjamin Montagne,Audrey Salles,Miria Ricchetti
View all publications
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