Rabbit Polyclonal CSB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.
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- Cell discovery 9:1072023Heat shock protein DNAJA2 regulates transcription-coupled repair by triggering CSB degradation via chaperone-mediated autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaping Huang,Liya Gu,Guo-Min LiPubMed 37907457
- Nature communications 13:9742022Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamrata Kumar,Arjan F Theil,Vera Roginskaya,Yasmin Ali,Michael Calderon,Simon C Watkins,Ryan P Barnes,Patricia L Opresko,Alex Pines,Hannes Lans,Wim Vermeulen,Bennett Van HoutenPubMed 35190564
- Genes 12:2021Identification and Characterization of a Novel Recurrent Variant in Patients with a Severe Form of Cockayne Syndrome B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhouloud Zayoud,Ichraf Kraoua,Asma Chikhaoui,Nadège Calmels,Sami Bouchoucha,Cathy Obringer,Clément Crochemore,Dorra Najjar,Sinda Zarrouk,Najoua Miladi,Vincent Laugel,Miria Ricchetti,Ilhem Turki,Houda Yacoub-YoussefPubMed 34946871
- Frontiers in cell and developmental biology 9:7278362021Functions of the CSB Protein at Topoisomerase 2 Inhibitors-Induced DNA Lesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFranciele Faccio Busatto,Sofiane Y Mersaoui,Yilun Sun,Yves Pommier,Jean-Yves Masson,Jenifer SaffiPubMed 34746125
- Molecular cell 80:1013-1024.e62020A Surge of DNA Damage Links Transcriptional Reprogramming and Hematopoietic Deficit in Fanconi Anemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Shen,Rui Wang,Moon Jong Kim,Qianghua Hu,Chih-Chao Hsu,Jun Yao,Naeh Klages-Mundt,Yanyan Tian,Erica Lynn,Thomas F Brewer,Yilei Zhang,Banu Arun,Boyi Gan,Michael Andreeff,Shunichi Takeda,Junjie Chen,Jae-Il Park,Xiaobing Shi,Christopher J Chang,Sung Yun Jung,Jun Qin,Lei LiPubMed 33338401
- PLoS genetics 16:e10085552020XPF-ERCC1 protects liver, kidney and blood homeostasis outside the canonical excision repair pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLee Mulderrig et. al.PubMed 32271760
- Nature communications 10:55762019CSB promoter downregulation via histone H3 hypoacetylation is an early determinant of replicative senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClément Crochemore et. al.PubMed 31811121
- Nucleic acids research 48:1285-13002019An R-loop-initiated CSB-RAD52-POLD3 pathway suppresses ROS-induced telomeric DNA breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Tan,Meihan Duan,Tribhuwan Yadav,Laiyee Phoon,Xiangyu Wang,Jia-Min Zhang,Lee Zou,Li LanPubMed 31777915
- BMC cancer 19:9482019CSB affected on the sensitivity of lung cancer cells to platinum-based drugs through the global decrease of let-7 and miR-29.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenbang Yang et. al.PubMed 31615563
- Nucleic acids research 47:8548-85622019Cockayne syndrome group B deficiency reduces H3K9me3 chromatin remodeler SETDB1 and exacerbates cellular aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong-Hyuk Lee,Tyler G Demarest,Mansi Babbar,Edward W Kim,Mustafa N Okur,Supriyo De,Deborah L Croteau,Vilhelm A BohrPubMed 31276581
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 46% PBS, 2.4% Tris, 1.5% Glycine, 0.04% EGTA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link Q03468
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Essential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed:16246722, PubMed:20541997, PubMed:26620705). Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA (PubMed:15548521). Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function (PubMed:25820262). Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle (PubMed:25820262). Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing premature entry of cells into mitosis following the induction of DNA DSBs (PubMed:25820262). Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function. Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR (PubMed:29203878). Required for stable recruitment of ELOA and CUL5 to DNA damage sites (PubMed:28292928). Involved in UV-induced translocation of ERCC8 to the nuclear matrix (PubMed:26620705). Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis (PubMed:24874740).
Alternative names
CSB, ERCC6, DNA excision repair protein ERCC-6, ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB
Recommended products
Rabbit Polyclonal CSB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 46% PBS, 2.4% Tris, 1.5% Glycine, 0.04% EGTA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link Q03468
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-CSB antibody (ab96089)
7.5% SDS Page
All lanes: Western blot - Anti-CSB antibody (ab96089) at 1/500 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Predicted band size: 168 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CSB antibody (ab96089)
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab96089 antibody at 1/200 dilution. Lower image merged with DNA probe.
Western blot - Anti-CSB antibody (ab96089)
Western blot: Anti-ERCC6 antibody (ab96089) staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab96089 was shown to bind specifically to ERCC6. A band was observed at 180 kDa in wild-type A549 cell lysates with no signal observed at this size in ERCC6 knockout cell line. To generate this image, wild-type and ERCC6 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CSB antibody (ab96089) at 1/500 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: ERCC6 knockout A549 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: PC-3 Cytoplasmic Fraction cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 180 kDa
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