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Knockout Tested Rabbit Recombinant Monoclonal CSB antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

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Images

Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (AB316745), expandable thumbnail
  • Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (AB316745), expandable thumbnail
  • Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (AB316745), expandable thumbnail
  • Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (AB316745), expandable thumbnail
  • Immunoprecipitation - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (AB316745), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Essential factor involved in transcription-coupled nucleotide excision repair which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes (PubMed:16246722, PubMed:20541997, PubMed:26620705). Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA (PubMed:15548521). Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function (PubMed:25820262). Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle (PubMed:25820262). Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing premature entry of cells into mitosis following the induction of DNA DSBs (PubMed:25820262). Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function. Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR (PubMed:29203878). Required for stable recruitment of ELOA and CUL5 to DNA damage sites (PubMed:28292928). Involved in UV-induced translocation of ERCC8 to the nuclear matrix (PubMed:26620705). Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis (PubMed:24874740).

Alternative names

CSB, ERCC6, DNA excision repair protein ERCC-6, ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CSB antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27019-44
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316745 is the carrirer-free version of Anti-CSB antibody [EPR27019-44] ab316744.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745), expandable thumbnail

    Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745)

    This data was developed using the same antibody clone in a different buffer formulation (abAB316744).

    Western blot: Anti-ERCC6 antibody [EPR27019-44] (Anti-CSB antibody [EPR27019-44] ab316744) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, Anti-CSB antibody [EPR27019-44] ab316744 was shown to bind specifically to ERCC6. A band was observed at 180 kDa in wild-type A549 cell lysates with no signal observed at this size in ERCC6 knockout cell line. To generate this image, wild-type and ERCC6 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-CSB antibody [EPR27019-44] (Anti-CSB antibody [EPR27019-44] ab316744) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: ERCC6 knockout A549 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: PC-3 Cytoplasmic Fraction cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 180 kDa

  • Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745), expandable thumbnail

    Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745)

    This data was developed using Anti-CSB antibody [EPR27019-44] ab316744, the same antibody clone in a different buffer formulation.

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-CSB antibody [EPR27019-44] (Anti-CSB antibody [EPR27019-44] ab316744) at 1/1000 dilution

    Lane 1: U-2OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745), expandable thumbnail

    Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745)

    This data was developed using Anti-CSB antibody [EPR27019-44] ab316744, the same antibody clone in a different buffer formulation.

    This antibody was tested on fresh and frozen HeLa and 293T Whole Cell Lysate. The Lysates in lanes 3-4 were freshly made and used for Western Blotting immediately to minimize protein degradation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    Exposure time: Lane 1-2: 103 seconds; Lane 3-4: 180 seconds

    All lanes: Western blot - Anti-CSB antibody [EPR27019-44] (Anti-CSB antibody [EPR27019-44] ab316744) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: HeLa whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: 293T whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 124 kDa

  • Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745), expandable thumbnail

    Western blot - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745)

    This data was developed using Anti-CSB antibody [EPR27019-44] ab316744, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-CSB antibody [EPR27019-44] (Anti-CSB antibody [EPR27019-44] ab316744) at 1/1000 dilution

    Lane 1: Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg with 5% NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting ERCC6 whole cell lysate at 40 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 124 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745), expandable thumbnail

    Immunoprecipitation - Anti-CSB antibody [EPR27019-44] - BSA and Azide free (ab316745)

    This data was developed using Anti-CSB antibody [EPR27019-44] ab316744, the same antibody clone in a different buffer formulation.

    CSB was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate with Anti-CSB antibody [EPR27019-44] ab316744 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CSB antibody [EPR27019-44] ab316744 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate

    Lane 2: Anti-CSB antibody [EPR27019-44] ab316744 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CSB antibody [EPR27019-44] ab316744 in HeLa whole cell fresh lysate

    All lanes: Immunoprecipitation - Anti-CSB antibody [EPR27019-44] (Anti-CSB antibody [EPR27019-44] ab316744) at 1/30 dilution

    All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell fresh lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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