Anti-CSC1 antibody [EPR19814] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395)

This data was developed using ab203486, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red).

Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC.

The nuclear counter stain is DAPI (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395)

This data was developed using ab203486, the same antibody clone in a different buffer formulation.

CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse cerebellum lysate 10 μg (Input).
Lane 2 : ab203486 IP in mouse cerebellum lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203486 in mouse cerebellum lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

Note : The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

All lanes:

Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (<a href='/en-us/products/primary-antibodies/csc1-antibody-epr19814-ab203486'>ab203486</a>)

Predicted band size: 93 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395)

This data was developed using ab203486, the same antibody clone in a different buffer formulation.

CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Rat cerebellum lysate 10 μg (Input).
Lane 2 : ab203486 IP in rat cerebellum lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab203486 in rat cerebellum lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

Note : The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

All lanes:

Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (<a href='/en-us/products/primary-antibodies/csc1-antibody-epr19814-ab203486'>ab203486</a>)

Predicted band size: 93 kDa

false

• WB

Supplier Data

Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395)

This data was developed using ab203486, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 3 minutes; Lanes 2-4 : 10 seconds.

To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.

All lanes:

Western blot - Anti-CSC1 antibody [EPR19814] (<a href='/en-us/products/primary-antibodies/csc1-antibody-epr19814-ab203486'>ab203486</a>) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Rat cerebellum tissue lysate at 20 µg

Lane 4:

Rat cerebral cortex tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

• WB

Collaborator

Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395)

This data was developed using ab203486, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSC1 antibody [EPR19814] (<a href='/en-us/products/primary-antibodies/csc1-antibody-epr19814-ab203486'>ab203486</a>) at 1/400 dilution

Lane 1:

CSC1 KO (-/-) mouse brain lysate

Lane 2:

CSC1 WT (+/+) mouse brain lysate

Lane 3:

CSC1 heterozygous (+/-) mouse brain lysate

Secondary

All lanes:

Goat Anti-Mouse IgG (H+L) HRP at 1/15000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 30s

The Images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University, MARC.