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Rabbit Recombinant Monoclonal CSC1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395), expandable thumbnail
  • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395), expandable thumbnail
  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395), expandable thumbnail
  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (AB251395), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWB
Human
Tested
Expected
Expected
Mouse
Expected
Tested
Tested
Rat
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Acts as an osmosensitive cation channel preferentially activated upon hypotonic stress (PubMed:24503647, PubMed:35718349). Enriched in mitochondria-ER contact sites where it may regulate the metabolite flux and organelles' morphologies in response to osmotic changes. In particular may regulate mitochondrial motility and function in motor neuron axons (PubMed:35718349). Required for the functional integrity of the kidney glomerular filtration barrier.

Alternative names

C14orf171, CSC1, TMEM63C, Calcium permeable stress-gated cation channel 1, Transmembrane protein 63C

Recommended products

Rabbit Recombinant Monoclonal CSC1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR19814
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251395 is the carrier-free version of Anti-CSC1 antibody [EPR19814] ab203486.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395), expandable thumbnail

    Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

    This data was developed using Anti-CSC1 antibody [EPR19814] ab203486, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lanes 2-4: 10 seconds.

    To avoid the formation of CSC1 high molecule weight aggregates the lysates in sample buffer were not boiled prior to WB testing.

    All lanes: Western blot - Anti-CSC1 antibody [EPR19814] (Anti-CSC1 antibody [EPR19814] ab203486) at 1/1000 dilution

    Lane 1: Mouse cerebral cortex tissue lysate at 20 µg

    Lane 2: Mouse cerebellum tissue lysate at 20 µg

    Lane 3: Rat cerebellum tissue lysate at 20 µg

    Lane 4: Rat cerebral cortex tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 93 kDa

    Observed band size: 93 kDa

  • Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395), expandable thumbnail
    The Images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University, MARC.

    Western blot - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

    This data was developed using Anti-CSC1 antibody [EPR19814] ab203486, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CSC1 antibody [EPR19814] (Anti-CSC1 antibody [EPR19814] ab203486) at 1/400 dilution

    Lane 1: CSC1 KO (-/-) mouse brain lysate

    Lane 2: CSC1 WT (+/+) mouse brain lysate

    Lane 3: CSC1 heterozygous (+/-) mouse brain lysate

    Secondary

    All lanes: Goat Anti-Mouse IgG (H+L) HRP at 1/15000 dilution

    Predicted band size: 93 kDa

    Observed band size: 93 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395), expandable thumbnail

    Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

    This data was developed using Anti-CSC1 antibody [EPR19814] ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of mouse cerebellum lysate with Anti-CSC1 antibody [EPR19814] ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-CSC1 antibody [EPR19814] ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution. Lane 1: Mouse cerebellum lysate 10 μg (Input). Lane 2: Anti-CSC1 antibody [EPR19814] ab203486 IP in mouse cerebellum lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CSC1 antibody [EPR19814] ab203486 in mouse cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

    All lanes: Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (Anti-CSC1 antibody [EPR19814] ab203486)

    Predicted band size: 93 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

    This data was developed using Anti-CSC1 antibody [EPR19814] ab203486, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HA-mCSC1 transfected HEK-293T (Human epithelial cell line from embryonic kidney) cells labeling CSC1 with Anti-CSC1 antibody [EPR19814] ab203486 at 1/150 dilution (green). Counterstained with Anti-HA antibody at 1/500 dilution (red). Positive staining on HA-mCSC1 transfected HEK-293T cells. The images were kindly provided by our collaborator Dr. Yun Shi, Nanjing University MARC. The nuclear counter stain is DAPI (blue).

  • Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395), expandable thumbnail

    Immunoprecipitation - Anti-CSC1 antibody [EPR19814] - BSA and Azide free (ab251395)

    This data was developed using Anti-CSC1 antibody [EPR19814] ab203486, the same antibody clone in a different buffer formulation.CSC1 was immunoprecipitated from 0.35 mg of rat cerebellum lysate with Anti-CSC1 antibody [EPR19814] ab203486 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-CSC1 antibody [EPR19814] ab203486 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution. Lane 1: Rat cerebellum lysate 10 μg (Input). Lane 2: Anti-CSC1 antibody [EPR19814] ab203486 IP in rat cerebellum lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CSC1 antibody [EPR19814] ab203486 in rat cerebellum lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds. Note: The sample loaded onto the input lane is not boiled to avoid formation of CSC1 high molecule weight aggregates.

    All lanes: Immunoprecipitation - Anti-CSC1 antibody [EPR19814] (Anti-CSC1 antibody [EPR19814] ab203486)

    Predicted band size: 93 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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