AB200663

Anti-CSDE1/NRU antibody [EPR17413]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal CSDE1/NRU antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

D1S155E, KIAA0885, NRU, UNR, CSDE1, Cold shock domain-containing protein E1, N-ras upstream gene protein, Protein UNR

7 Images

Supplier Data

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/1000 dilution (Panel A) or ab201688 at 1/1000 dilution (Panel B). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab200663 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 min (Panel A); 10 seconds (Panel B).

All lanes:

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Predicted band size: 89 kDa

false

Supplier Data

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab200663 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (ab200663)

Predicted band size: 89 kDa,93 kDa

false

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

All lanes:

MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

The HeLa cell fractions lysate set is ab168542 from Abcam.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/2000 dilution

Lane 1:

HeLa cytosolic fraction at 10 µg

Lane 2:

HeLa membrane (mitochondrial) fraction at 10 µg

Lane 3:

HeLa nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (ab200663) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 1min

Carrier free

Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17413

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/140", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein involved in translationally coupled mRNA turnover (PubMed : 11051545, PubMed : 15314026). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed : 11051545, PubMed : 15314026). Required for efficient formation of stress granules (PubMed : 29395067).. (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA.

See full target information CSDE1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102585 PubMed36223838

2022

Tick-borne encephalitis virus capsid protein induces translational shutoff as revealed by its structural-biological analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Selinger,Radim Novotný,Jakub Sýs,Justin A Roby,Hana Tykalová,Ganji Sri Ranjani,Marie Vancová,Kateřina Jaklová,Filip Kaufman,Marshall E Bloom,Zbyněk Zdráhal,Libor Grubhoffer,Jade K Forwood,Richard Hrabal,Michaela Rumlová,Ján Štěrba

Nucleic acids research 46:11575-11591 PubMed30247708

2018

Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.

Applications

Unspecified application

Species

Unspecified reactive species

Evan P Booy,Ewan Ks McRae,Peyman Ezzati,Taegi Choi,Daniel Gussakovsky,Sean A McKenna

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CSDE1/NRU antibody [EPR17413]

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (AB200663)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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