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AB251319

Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free

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Rabbit Recombinant Monoclonal CSDE1/NRU antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

D1S155E, KIAA0885, NRU, UNR, CSDE1, Cold shock domain-containing protein E1, N-ras upstream gene protein, Protein UNR

7 Images
Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)
  • IP

Supplier Data

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)

This data was developed using ab200663, the same antibody clone in a different buffer formulation.

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/1000 dilution (Panel A) or ab201688 at 1/1000 dilution (Panel B). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab200663 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 min (Panel A); 10 seconds (Panel B).

All lanes:

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (<a href='/en-us/products/primary-antibodies/csde1-nru-antibody-epr17413-ab200663'>ab200663</a>)

Predicted band size: 89 kDa

false

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)
  • IP

Supplier Data

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)

This data was developed using ab200663, the same antibody clone in a different buffer formulation.

CSDE1/NRU was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab200663 at 1/140 dilution. Western blot was performed from the immunoprecipitate using ab200663 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input). Lane 2 : ab200663 IP in HeLa whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200663 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17413] (<a href='/en-us/products/primary-antibodies/csde1-nru-antibody-epr17413-ab200663'>ab200663</a>)

Predicted band size: 89 kDa,93 kDa

false

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)
  • WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)

This data was developed using ab200663, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (<a href='/en-us/products/primary-antibodies/csde1-nru-antibody-epr17413-ab200663'>ab200663</a>) at 1/2000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)
  • WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)

This data was developed using ab200663, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (<a href='/en-us/products/primary-antibodies/csde1-nru-antibody-epr17413-ab200663'>ab200663</a>) at 1/1000 dilution

All lanes:

MCF-7 (Human breast adenocarcinoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)
  • WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)

This data was developed using ab200663, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The HeLa cell fractions lysate set is ab168542 from Abcam.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (<a href='/en-us/products/primary-antibodies/csde1-nru-antibody-epr17413-ab200663'>ab200663</a>) at 1/2000 dilution

Lane 1:

HeLa cytosolic fraction at 10 µg

Lane 2:

HeLa membrane (mitochondrial) fraction at 10 µg

Lane 3:

HeLa nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)
  • WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)

This data was developed using ab200663, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (<a href='/en-us/products/primary-antibodies/csde1-nru-antibody-epr17413-ab200663'>ab200663</a>) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 3min

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)
  • WB

Supplier Data

Western blot - Anti-CSDE1/NRU antibody [EPR17413] - BSA and Azide free (AB251319)

This data was developed using ab200663, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CSDE1/NRU antibody [EPR17413] (<a href='/en-us/products/primary-antibodies/csde1-nru-antibody-epr17413-ab200663'>ab200663</a>) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 89 kDa

Observed band size: 89 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17413

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251319 is the carrier-free version of ab200663.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein involved in translationally coupled mRNA turnover (PubMed : 11051545, PubMed : 15314026). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed : 11051545, PubMed : 15314026). Required for efficient formation of stress granules (PubMed : 29395067).. (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA.
See full target information CSDE1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com