Rabbit Recombinant Monoclonal CSDE1/NRU antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67649.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99104.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149)")
![Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunoprecipitation-img5998.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149)")
![Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunocytochemistry-immunofluorescence-img122957.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149)")
![Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunocytochemistry-immunofluorescence-img108640.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (AB236149)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Predicted | Expected | Predicted | Predicted |
| Rat | Predicted | Expected | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
RNA-binding protein involved in translationally coupled mRNA turnover (PubMed:11051545, PubMed:15314026). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545, PubMed:15314026). Required for efficient formation of stress granules (PubMed:29395067).(Microbial infection) Required for internal initiation of translation of human rhinovirus RNA.
Alternative names
Cold shock domain-containing protein E1, N-ras upstream gene protein, Protein UNR, D1S155E, UNR, NRU, KIAA0885, CSDE1
Recommended products
Rabbit Recombinant Monoclonal CSDE1/NRU antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
Alternative names
Cold shock domain-containing protein E1, N-ras upstream gene protein, Protein UNR, D1S155E, UNR, NRU, KIAA0885, CSDE1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR17414
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab236149 is the carrier-free version of Anti-CSDE1/NRU antibody [EPR17414] ab201688.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67649.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CSDE1/NRU with Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on Human breast carcinoma tissue is observed.
Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CSDE1/NRU antibody [EPR17414] ab201688).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99104.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CSDE1/NRU with Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasmic staining on Human kidney tissue is observed.
Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CSDE1/NRU antibody [EPR17414] ab201688).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunoprecipitation-img5998.jpg "Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)")
Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
CSDE1/NRU was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/1500 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1500 dilution.
Lane 1: K562 whole cell extract 10 μg (Input).
Lane 2: Anti-CSDE1/NRU antibody [EPR17414] ab201688 IP in K562 whole cell extract.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab20688 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 second
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CSDE1/NRU antibody [EPR17414] ab201688).
All lanes: Immunoprecipitation - Anti-CSDE1/NRU antibody [EPR17414] (Anti-CSDE1/NRU antibody [EPR17414] ab201688)
Predicted band size: 89 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunocytochemistry-immunofluorescence-img122957.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)")
Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling CSDE1/NRU with Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasmic staining on HeLa cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CSDE1/NRU antibody [EPR17414] ab201688).
![Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149), expandable thumbnail](https://content.abcam.com/products/images/csde1-nru-antibody-epr17414-bsa-and-azide-free-ab236149--immunocytochemistry-immunofluorescence-img108640.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)")
Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] - BSA and Azide free (ab236149)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling CSDE1/NRU with Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. Anti-CSDE1/NRU antibody [EPR17414] ab201688 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CSDE1/NRU antibody [EPR17414] ab201688).
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