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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Goat Polyclonal CSF-1-R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CSF1R aa 700-750.
IgG
Goat
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Cat | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.1-0.5 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cat | Dilution info - | Notes - |
Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).
Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R, FMS, CSF1R
Goat Polyclonal CSF-1-R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CSF1R aa 700-750.
Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R, FMS, CSF1R
IgG
Goat
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Liquid
Polyclonal
Affinity purification Immunogen
ab223642 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Primary incubations were for 1 hour.
Lane 1: Western blot - Anti-CSF-1-R antibody (AB223642) at 0.5 µg/mL
Lane 2: Western blot - Anti-CSF-1-R antibody (AB223642) at 1 mg/mL
Lane 3: Anti-MYC tag at 1/1000 dilution
Lanes 1 and 3: HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human CSF-1-R with C-terminal MYC tag (in RIPA buffer) at 10 µg
Lane 2: Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate (in RIPA buffer) at 10 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 200 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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