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Goat Polyclonal CSF-1-R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CSF1R aa 700-750.

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Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human CSF1R aa 700-750. The exact immunogen used to generate this antibody is proprietary information. Database link P07333

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Cat
Predicted

Tested
Tested

Species

Human

Dilution info

0.1-0.5 µg/mL

Notes

A 1 hour primary incubation is recommended for this product.

Predicted
Predicted

Species

Mouse, Rat, Cat

Dilution info

-

Notes

-

Target data

Function

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed:20504948, PubMed:30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed:30982608).

Alternative names

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Goat Polyclonal CSF-1-R antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CSF1R aa 700-750.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human CSF1R aa 700-750. The exact immunogen used to generate this antibody is proprietary information. Database link P07333
Purification technique

Affinity purification Immunogen

Concentration
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Purification notes

ab223642 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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1 product image

  • Western blot - Anti-CSF-1-R antibody (ab223642), expandable thumbnail

    Western blot - Anti-CSF-1-R antibody (ab223642)

    Primary incubations were for 1 hour.

    Lane 1: Western blot - Anti-CSF-1-R antibody (AB223642) at 0.5 µg/mL

    Lane 2: Western blot - Anti-CSF-1-R antibody (AB223642) at 1 mg/mL

    Lane 3: Anti-MYC tag at 1/1000 dilution

    Lanes 1 and 3: HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human CSF-1-R with C-terminal MYC tag (in RIPA buffer) at 10 µg

    Lane 2: Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate (in RIPA buffer) at 10 µg

    Developed using the ECL technique.

    Predicted band size: 108 kDa

    Observed band size: 200 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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