AB229188

Anti-CSF-1-R antibody [EPR20754]

20ul selling size
RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal CSF-1-R antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CD115, FMS, CSF1R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, Proto-oncogene c-Fms, CSF-1-R, CSF-1R, M-CSF-R

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (AB229188)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages infiltrating human lung cancer is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic and membranous staining of human non-Hodgkin lymphoma cells is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages of human tonsil (PMID : 26066800) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR20754] (AB229188)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) labelling CSF-1-R with ab229188 at 1/100 (10.55 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing membrane and cytoplasmic staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : Raji.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab229188 at a 1/100 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab7291 at a 1/1000 dilution followed by ab150081 at a 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] (AB229188)

CSF-1-R was immunoprecipitated from 0.35 mg of human tonsil lysate with ab229188 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229188 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human tonsil lysate 10 μg (Input).
Lane 2 : ab229188 IP in human tonsil lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229188 in human tonsil lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] (ab229188)

Predicted band size: 108 kDa

Observed band size: 108 kDa

false

Supplier Data

Western blot - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Blocking/dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-CSF-1-R antibody [EPR20754] (ab229188) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa

true

Exposure time: 3min

Carrier free

Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20754

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine-protein kinase that acts as a cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding, including the ERK1/2 and the JNK pathway (PubMed : 20504948, PubMed : 30982609). Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. In the central nervous system, may play a role in the development of microglia macrophages (PubMed : 30982608).

See full target information CSF1R

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Translational cancer research 14:3319-3335 PubMed40687256

2025

Hepatitis B virus core/capsid protein induces hepatocellular carcinoma progression via long non-coding RNA KCNQ1OT1/miR-335-5p/CDC7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Xinyi Kong,Xin Yu,Jie Qiao,Kaming Yang,Zhenlin Li,Yiling Wu,Wanwen Lao,Yuetong Li,Yiqi Lin,Yanping Zhang,Changlong Bi,Aixia Zhai

The FEBS journal 291:5214-5235 PubMed39434428

2024

TBK1 is involved in M-CSF-induced macrophage polarization through mediating the IRF5/IRF4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Li,Le Ji,Chang Liu,Juanjuan Li,Di Wen,Zhongyao Li,Lishuang Yu,Moran Guo,Shaoran Zhang,Weisong Duan,Le Yi,Yue Bi,Hui Bu,Chunyan Li,Yakun Liu

Thoracic cancer 14:254-266 PubMed36448247

2022

RNA m6A methyltransferase METTL14 promotes the procession of non-small cell lung cancer by targeted CSF1R.

Applications

Unspecified application

Species

Unspecified reactive species

Siying Ren,Ying Xiao,Lulu Yang,Yan Hu

Clinical cancer research : an official journal of the American Association for Cancer Research 27:5519-5527 PubMed34321280

2021

A Phase I Study of the Combination of Pexidartinib and Sirolimus to Target Tumor-Associated Macrophages in Unresectable Sarcoma and Malignant Peripheral Nerve Sheath Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Gulam A Manji,Brian A Van Tine,Shing M Lee,Alexander G Raufi,Ilenia Pellicciotta,Angela C Hirbe,Jaya Pradhan,Andrew Chen,Raul Rabadan,Gary K Schwartz

Frontiers in cell and developmental biology 9:641133 PubMed33816491

2021

Variants Affecting the C-Terminal of CSF1R Cause Congenital Vertebral Malformation Through a Gain-of-Function Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Liu,Sen Zhao,Zihui Yan,Lina Zhao,Jiachen Lin,Shengru Wang,Yuchen Niu,Xiaoxin Li,Guixing Qiu,Terry Jianguo Zhang,Zhihong Wu,Nan Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CSF-1-R antibody [EPR20754]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Western blot - Anti-CSF-1-R antibody [EPR20754] (AB229188)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

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