Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (AB231074)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages infiltrating human lung cancer. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (AB231074)

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic and membranous staining of human non-Hodgkin lymphoma cells is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (AB231074)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CSF-1-R with ab229188 at 1/250 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use. Cytoplasmic staining in macrophages of human tonsil (PMID : 26066800) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (AB231074)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) labelling CSF-1-R with ab229188 at 1/100 (10.55 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing membrane and cytoplasmic staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : Raji.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab229188 at a 1/100 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab7291 at a 1/1000 dilution followed by ab150081 at a 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (AB231074)

CSF-1-R was immunoprecipitated from 0.35 mg of human tonsil tissue lysate with ab229188 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229188 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human tonsil lysate 10 μg (Input).
Lane 2 : ab229188 IP in human tonsil lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab229188 in human tonsil lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

All lanes:

Immunoprecipitation - Anti-CSF-1-R antibody [EPR20754] (<a href='/en-us/products/primary-antibodies/csf-1-r-antibody-epr20754-ab229188'>ab229188</a>)

Predicted band size: 108 kDa

Observed band size: 108 kDa

false

• WB

Supplier Data

Western blot - Anti-CSF-1-R antibody [EPR20754] - BSA and Azide free (AB231074)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229188).

Blocking/dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-CSF-1-R antibody [EPR20754] (<a href='/en-us/products/primary-antibodies/csf-1-r-antibody-epr20754-ab229188'>ab229188</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 108 kDa

Observed band size: 108 kDa

true

Exposure time: 3min